CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

SC Hyde, IA Pringle, S Abdullah, AE Lawton… - Nature …, 2008 - nature.com
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with
an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief …

Progress and prospects: the design and production of plasmid vectors

DR Gill, IA Pringle, SC Hyde - Gene therapy, 2009 - nature.com
Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene
transfer. In this review, we discuss principles of pDNA design and production including the …

Structural model of ATP-binding proteing associated with cystic fibrosis, multidrug resistance and bacterial transport

SC Hyde, P Emsley, MJ Hartshorn, MM Mimmack… - Nature, 1990 - nature.com
Abstract THE ATP-binding cassette (ABC) superfamily of transport systems now includes
over thirty proteins that share extensive sequence similarity and domain organization …

A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria

CF Higgins, ID Hiles, GPC Salmond, DR Gill… - Nature, 1986 - nature.com
Many biological processes are coupled to ATP hydrolysis. We describe here a class of
closely related ATP-binding proteins, from several bacterial species, which are associated …

[HTML][HTML] Repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis: a randomised, double-blind, placebo-controlled, phase 2b trial

EWFW Alton, DK Armstrong, D Ashby… - The Lancet …, 2015 - thelancet.com
Background Lung delivery of plasmid DNA encoding the CFTR gene complexed with a
cationic liposome is a potential treatment option for patients with cystic fibrosis. We aimed to …

[HTML][HTML] Identification of LZTFL1 as a candidate effector gene at a COVID-19 risk locus

DJ Downes, AR Cross, P Hua, N Roberts… - Nature …, 2021 - nature.com
The severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) disease (COVID-19)
pandemic has caused millions of deaths worldwide. Genome-wide association studies …

Volume-regulated chloride channels associated with the human multidrug-resistance P-glycoprotein

MA Valverde, M DÍaz, FV Sepúlveda, DR Gill, SC Hyde… - Nature, 1992 - nature.com
EXPRESSION of P-glycoprotein, the product of the MDR1 gene, confers multidrug
resistance on cell lines and human tumours (reviewed in refs 1, 2). P-glycoprotein (relative …

Correction of the ion transport defect in cystic fibrosis transgenic mice by gene therapy

SC Hyde, DR Gill, CF Higgins, AEO Trezise… - Nature, 1993 - nature.com
CYSTIC fibrosis (CF) is a lethal inherited disorder affecting about 1 in 2,000 Caucasians.
The major cause of morbidity is permanent lung damage resulting from ion transport …

Lessons learned from lung and liver in-vivo gene therapy: implications for the future

J van Haasteren, SC Hyde, DR Gill - Expert opinion on biological …, 2018 - Taylor & Francis
Introduction: Ex-vivo gene therapy has had significant clinical impact over the last couple of
years and in-vivo gene therapy products are being approved for clinical use. Gene therapy …

Changes in physiological, functional and structural markers of cystic fibrosis lung disease with treatment of a pulmonary exacerbation

AR Horsley, JC Davies, RD Gray, KA Macleod… - Thorax, 2013 - thorax.bmj.com
Background Clinical trials in cystic fibrosis (CF) have been hindered by the paucity of well
characterised and clinically relevant outcome measures. Aim To evaluate a range of …