Abstract
The in vitro biosynthesis of isoprene from DL-mevalonate in the cytosolic fraction of rat liver is described. Evidence is provided suggesting a non-enzymatic formation of isoprene from isopentenyl pyrophosphate and/or dimethylallyl pyrophosphate. Furthermore, the data establish an alternate fate of the mevalonate carbon skeleton providing the first evidence that breath isoprene is linked to cholesterol biosynthesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Breath Tests
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Butadienes / biosynthesis*
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Cytosol / metabolism
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Female
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Hemiterpenes*
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In Vitro Techniques
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Liver / metabolism*
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Magnesium / metabolism
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Male
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Mevalonic Acid / metabolism*
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Organophosphorus Compounds / metabolism
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Pentanes*
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Rats
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Rats, Inbred Strains
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Time Factors
Substances
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Butadienes
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Hemiterpenes
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Organophosphorus Compounds
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Pentanes
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isoprene
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3,3-dimethylallyl pyrophosphate
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Adenosine Triphosphate
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Magnesium
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Mevalonic Acid