A native soluble form of CTLA-4

Cell Immunol. 2000 May 1;201(2):144-53. doi: 10.1006/cimm.2000.1649.

Abstract

CTLA-4 is an immunoregulatory receptor expressed on the surface of activated T and B lymphocytes. The counterreceptors for CTLA-4 are the B7 family molecules. We describe alternatively spliced mRNAs expressed in hematolymphoid tissues of humans, mice, and rats that lack the transmembrane domain coded by exon 3 of the CTLA-4 gene. These alternate transcripts were detected by RT-PCR in B cells and resting T cells of both the CD4 and the CD8 phenotype. Activation of human blood mononuclear cells with PHA or anti-CD3 + anti-CD28 monoclonal antibodies appears to effect a decrease in the amount of the alternative transcript relative to the full-length transcript. Recombinant sCTLA-4 is a B7-binding protein and has immunomodulatory effects as measured by inhibition of the mixed leukocyte response. Human serum contains immunoreactive material consistent with a native soluble form of CTLA-4.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abatacept
  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Antigens, Differentiation / blood
  • Antigens, Differentiation / genetics*
  • B7-1 Antigen
  • B7-2 Antigen
  • Base Sequence
  • CTLA-4 Antigen
  • Cloning, Molecular
  • Female
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Immunoconjugates*
  • Lymphoid Tissue / immunology
  • Membrane Glycoproteins
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • RNA, Messenger / isolation & purification
  • Rats
  • Receptors, Immunologic / blood
  • Receptors, Immunologic / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Solubility

Substances

  • Antigens, CD
  • Antigens, Differentiation
  • B7-1 Antigen
  • B7-2 Antigen
  • CD86 protein, human
  • CTLA-4 Antigen
  • CTLA4 protein, human
  • Cd86 protein, mouse
  • Cd86 protein, rat
  • Ctla4 protein, mouse
  • Ctla4 protein, rat
  • Immunoconjugates
  • Membrane Glycoproteins
  • Membrane Proteins
  • RNA, Messenger
  • Receptors, Immunologic
  • Abatacept

Associated data

  • GENBANK/AF012711
  • GENBANK/AF012712
  • GENBANK/AF012713
  • GENBANK/U90270
  • GENBANK/U90271
  • GENBANK/U90273