Chest
Clinical InvestigationsCOPDPerforin Expression and Cytotoxic Activity of Sputum CD8+ Lymphocytes in Patients With COPD
Section snippets
Subjects
Seventy-one male subjects were studied: 36 current smokers with COPD, 25 non-COPD smokers with a similar smoking history to the patients with COPD, and 10 healthy nonsmokers (Table 1). The diagnosis of COPD was based on the European consensus criteria.2 All patients with COPD had been free of an acute exacerbation for at least 4 weeks preceding the study, and none had received antibiotics or corticosteroids (oral and inhaled) over the same period. Neither smokers nor healthy volunteers had a
Results
Demographic and spirometric data of the three groups of subjects are shown in Table 1. The patients with COPD exhibited significantly lower values of FEV1 (percentage of predicted) and FEV1/FVC (percentage) than non-COPD smokers, although the smoking history was similar. There were no significant differences in FEV1/FVC (percentage) and FEV1 (percentage) between non-COPD smokers and normal subjects. Change in FEV1 (percentage of predicted) after salbutamol was similar in the three groups (Table
Discussion
In agreement with other studies,1318 we also showed that it is possible to analyze sputum samples for lymphocyte cell surface markers by flow cytometry. The main findings of the present study were that CD8+ lymphocytes of patients with COPD showed increased cytotoxic activity and increased expression of intracellular perforin compared to smokers without COPD, but with similar smoking history, and healthy nonsmokers (normal subjects). It is well known that it is difficult to induce sputum
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Cited by (135)
Enhanced activation of circulating plasmacytoid dendritic cells in patients with Chronic Obstructive Pulmonary Disease and experimental smoking-induced emphysema
2018, Clinical ImmunologyCitation Excerpt :It has been reported that the accumulation of mDCs and pDCs in airways of patients with COPD [8,9,10,11]; however, the phenotype of circulating pDCs in patients with COPD and its role in CD8+ T cell-mediated immune response remain to be elucidated. Previous studies have shown that CD8+ T cells were accumulated in the airways, lung parenchyma and pulmonary arteries of COPD, through the expression of granzyme B, perforin and high levels of IFN-γ exerted cytotoxicity and pro-inflammatory activities, leading to the destruction of lung tissue [12,13,14,15]. In addition, the numbers of CD8+ T cells were significantly correlated with the degree of airflow limitation, which highlighted a substantial role of CD8+ T cells in the chronic inflammatory process of COPD [12,13].
EARLY versus MILD Chronic Obstructive Pulmonary Disease (COPD)
2018, Respiratory MedicineCOPD: Pathogenesis and Natural History
2015, Murray and Nadel's Textbook of Respiratory Medicine: Volume 1,2, Sixth Edition
This study was supported by an unrestricted grant from ASTRA Hellas.
Part of this study has been presented at the 11th Hellenic Thoracic Society annual meeting, Thessalonica, December 6–9, 2001, and as prize winner an extensive summary has been published in the Greek journal Pneumon (2002; 15:78).
This work has been awarded the national prize during the 12th European Respiratory Society meeting, Stockholm, Sweden, September 14–18, 2002.