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Clinical Investigations: Asthma/BronchodilatorsDetection of Cytokines and Their Cell Sources in Bronchial Biopsy Specimens From Asthmatic Patients: Relationship to Atopic Status, Symptoms, and Level of Airway Hyperresponsiveness
Section snippets
Subjects and Study Design
Thirty-three nonsmokers were selected for this study (11 women; mean age, 32 years [range, 18 to 45 years]). They were recruited among the patients of the Diagnostic Center for Respiratory and Allergic Diseases and from asthmatic subjects or healthy volunteers responding to advertisements. Twenty-eight subjects had a history of allergic or nonallergic asthma lasting for 2 years or longer. Five were healthy control subjects with no history of asthma or atopic diseases and with negative skin
Functional Assessment
Atopic and nonatopic asthmatic subjects were allocated into two distinct subgroups on the basis of symptom scores (Table 1). Asymptomatic patients were free of symptoms during the monitoring period and did not receive any medication. Patients with current symptoms of asthma had spontaneous airflow obstruction, as demonstrated by the diurnal and day-to-day variability of PEFR, and had higher levels of airway responsiveness to inhaled metha-choline than asymptomatic subjects (Table 1).
Immunohistochemistry and Immunoelectron Microscopy
Symptomatic
DISCUSSION
In our study, we confirm and extend previous observations indicating that the bronchial mucosa of asthmatic patients is infiltrated with increased numbers of activated eosinophils and T lymphocytes1, 2, 3, 4, 5, 6, 7,12,13 and that this occurs both in atopic and nonatopic patients.6,7 Infiltration of eosinophils and their activation may contribute to airflow obstruction and functional derangement, because the number of activated eosinophils was higher in asthmatic subjects with current symptoms
ACKNOWLEDGMENT
We thank all of the subjects who volunteered to participate in this study, S. Moretti for laboratory assistance, and A. Grossi for typing the manuscript.
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Supported by funds from the National Research Council, the Italian Association for Cancer Research, and CARIPLO.