Chest
ReviewProperties of the Mycobacterial Antigen Complex A60 and Its Applications to the Diagnosis and Prognosis of Tuberculosis
Section snippets
COMPOSITION AND PROPERTIES OF ANTIGEN A60 FROM BCG
When proteins from the cytoplasm of M bovis are separated by two-dimensional Immunoelectrophoresis, antigen 60 (A60) is the less mobile polymer;20 A60, the main thermostable component of old tuberculin (OT) and purified protein derivative of tuberculin (PPD),21 belongs to a family of major polymers (TMA or thermostable macromolecular antigens) of microorganisms from the genera, Corynebacterium, Mycobacterium, and Nocardia (CMN group).
A procedure for A60 purification has been described.22 This
SEROLOGIC ANALYSIS OF PULMONARY TUBERCULOSIS
The A60 ELISA has been used on human samples; a commercial diagnostic kit (Anda Biologicals; Strasbourg, France) was developed for this purpose. The first trials were carried out in our laboratory, in collaboration with the University Clinic at Mont Godinne. Three groups of control subjects were examined. Newborn children and tuberculin-negative healthy adults (controls) yielded negative reactions, while eight IgG-positive cases were found out of 124 tuberculin-negative nontuberculous pulmonary
INTERPRETATION OF SEROLOGIC DATA
The cited data indicate that mycobacterial proliferation, following either primary infection or reinfection, leads to the production of anti-A60 Ig, which decreases to possibly basal levels when the infectious process is controlled by therapy. Note that virtually all BCG-vaccinated tuberculin-positive healthy individuals have negative A60 ELISA values; however, if infection progresses, production of anti-A60 Ig can be detected in a substantial number of cases (up to 94 percent, according to
ANALYSIS OF CEREBROSPINAL FLUID IN TUBERCULOUS MENINGITIS
Recovery from tuberculous meningitis depends on early therapeutic intervention; however, early diagnosis is hampered by the paucity of mycobacteria in the cerebrospinal fluid and their long generation time. To solve this problem, an A60-based immunoblot technique has been developed. Accordingly, samples of cerebrospinal fluid were fractionated by isoelectric focusing on agarose gel plates and then transblotted to A60-loaded nitrocellulose sheets; the antigen-antibody complexes, after reaction
USE OF A60 AS A NEW TUBERCULIN
The main limitations of OT and PPD are (1) differences from batch to batch in the composition, (2) instability in a liquid form, and (3) imprecise standardization (subjective evaluation of tuberculin activity). Searches for new tuberculins are aimed at avoiding these shortcomings.39, 40 Since A60 is a component of OT and PPD21 and triggers delayed hypersensitivity reactions,27 its use as a reagent for cutaneous testing in tuberculosis has been envisaged. To avoid sensitization from repeated
CONCLUSIONS AND PERSPECTIVES
The present article focuses on three A60-based immunoassays for tuberculosis: (1) an ELISA procedure monitoring the evolution of the infectious process; (2) an immunoblot technique for the diagnosis of tuberculous meningitis; and (3) a cutaneous test for tuberculoimmunity. As indicated by the similarity of patterns with A60, whole mycobacterial antigens, and proliferating mycobacteria, the A60 complex is prominent among mycobacterial components in triggering cellular and humoral reactions in
ACKNOWLEDGMENT
The author is indebted to Dr. K. Willard-Gallo (ICP, Brussels) for correction of the manuscript, and to Dr. M. J. Gevaudan (Marseilles, France) for providing Figure 2 for publication.
REFERENCES (45)
- et al.
The relationship of delayed hypersensitivity to acquired antituberculous immunity: 1. tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice
Cell Immunol
(1970) Serodiagnosis of tuberculosis in perspective
Serodiagn Immunother
(1987)- et al.
Enzyme-linked immunosorbent assay (ELISA): a study of antibodies to M. tuberculosis in the IgG, IgA and IgM classes in tuberculosis, sarcoidosis and Crohn's disease
Tubercle
(1980) - et al.
ELISA test for antibodies against mycobacterial glycolipids
J Immunol Methods
(1980) - et al.
Elisa using M. tuberculosis antigen 5 of PPD for the serodiagnosis of tuberculosis
Chest
(1985) The immunology of mycobacterial infection
Crit Rev Microbiol
(1982)Relation between delayed hypersensitivity and immunity in tuberculosis
Am Rev Respir Dis
(1975)- et al.
Preliminary demonstration of human tuberculo-immunity in vitro
Infect Immun
(1981) The humoral immune response in tuberculosis: its nature, biological role and diagnostic usefulness
Adv Tuberc Res
(1984)- et al.
Mycobacterium
Bacteriol Rev
(1977)
Characterization and comparison of mycobacterial antigens by two-dimensional polyacrylamide gel electrophoresis
Infect Immun
Mycobacterial antigens: a review of their isolation, chemistry and immunological properties
Microbiol Rev
IgG antibody to purified protein derivative by ELISA in the diagnosis of pulmonary tuberculosis
Am Rev Respir Dis
Comparison of enzyme-linked immunosorbent assay and hemagglutination test using mycobacterial glycolipids
J Clin Microbiol
Serodiagnosis of tuberculosis using ELISA (enzyme-linked immuno-absorbent assay) or antibody to Mycobacterium tuberculosis antigen 5
Am Rev Respir Dis
Protein G-based enzyme-linked immunosorbent assay for anti MPB 70 antibodies in bovine tuberculosis
J Clin Microbiol
Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G direct against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis
Scand J Immunol
Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis
Infect Immun
Serodiagnosis of pulmonary tuberculosis in Argentina by enzyme-linked immunosorbent assay (ELISA) of IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative
Bull WHO
The antigens of Mycobacterium bovis strain BCG, studied by crossed Immunoelectrophoresis: a reference system
Scand J Immunol
Antigens of PPD, old tuberculin and autoclaved M. bovis BCG studied by crossed Immunoelectrophoresis
Am Rev Respir Dis
Preparation and properties of antigen 60 from Mycobacterium bovis BCG
Clin Exp Immunol
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2019, Journal of Cystic FibrosisPurification of modified mycobacterial A60 antigen by affinity chromatography and its use for rapid diagnostic tuberculosis infection
2011, Journal of Microbiological MethodsCitation Excerpt :There is a report of the use of a single antigen, the 38 kDa antigen, as a very good diagnostic marker (Bothamley and Rudd, 1994) but it still lacks sufficient sensitivity, especially for smear-negative individuals, where sensitivity is considerably lower (Stavri et al., 2003). Antigen 60, a thermo stable component of PPD, has also been used in the serodiagnosis of TB (Gupta et al., 1995; Cocito, 1991; Charpin et al., 1990). The sensitivity rate of antigen 60 for adults with active pulmonary TB is approximately 60 to 89%, with the rate lower for children and patients with extra-pulmonary TB (Charpin et al., 1990; Turneer et al., 1994).
Evaluation of the diagnostic value of measuring IgG, IgM, and IgA antibodies to mycobacterial A60 antigen in active tuberculosis
2010, Diagnostic Microbiology and Infectious DiseaseCitation Excerpt :It contains about 89 epitopes recognized by different antibodies. It is part of the group of antigenic complexes called the thermostable macromolecular antigens, which are highly conserved within the mycobacteria (Cocido, 1991). Distinguishing active TB from non-TB diseases in clinical practice using the ELISA IgG against mycobacterial A60 antigen has proven utility.
Chemotherapy and diagnosis of tuberculosis
2006, Respiratory MedicineThe value of pleural fluid anti-A60 IgM in BCG-vaccinated tuberculous pleurisy patients
2003, Clinical Microbiology and InfectionCitation Excerpt :Furthermore, it may be non-specific because of cross-reactivity with atypical mycobacterial proteins. Also, there are some studies reporting that the BCG vaccination status does not affect the rate of false-positive results of A60-based serologic tests among healthy volunteers [28,29]. This issue deserves further investigation.