Chest
Original ResearchCOPDMatrix Metalloproteinase-2 Protein in Lung Periphery Is Related to COPD Progression
Section snippets
Study Population
To quantify the expression of MMP-2, we collected lung tissue from 46 subjects undergoing surgery for appropriate clinical indications (ie, lung volume reduction surgery for the treatment of severe emphysema or lung resection for a solitary peripheral carcinoma). The subjects were categorized into the following four groups: smokers with severe COPD (GOLD stage III-IV [ie, FEV1/FVC ratio, < 70%; FEV1, < 50% predicted]; n = 10); smokers with mild/moderate COPD (GOLD stage I–II [ie, FEV1/FVC
Results
Table 1shows the clinical characteristics of the subjects examined. The complete analysis of clinical and functional parameters is reported in Web-only material.
As for MMP-2 immunoreactivity, a prominent staining was observed in alveolar macrophages and alveolar walls (Fig 1), as well as in peripheral airways, and pulmonary arterioles, where it was mainly associated with smooth muscle bundles. The percentage of MMP-2+ macrophages differed significantly among the four groups of subjects examined
Discussion
This study shows that in patients with COPD there is a marked up-regulation of MMP-2 in the lung periphery that is related to the degree of lung function impairment and emphysema. These results suggest that MMP-2 may be a key mediator of the mechanisms leading to lung tissue destruction in patients with severe COPD.
A number of animal models have illustrated the potential role of MMPs in the development of emphysema. In guinea pigs, exposure to cigarette smoke produces emphysematous changes that
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This research was funded by the University of Padova, the Italian Ministry of University and Research (MUR), and by a grant from the European Respiratory Society (to Dr. Baraldo).
The authors have reported to the ACCP that no significant conflicts of interest exist with any companies/organizations whose products or services may be discussed in this article.