1887

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Volume 36, Issue 5

Production and characterisation of mouse monoclonal antibodies reactive with the lipopolysaccharide core of Free

Abstract

Summary

Monoclonal antibodies (MAbs) to the core antigen region of lipopolysaccharide (LPS) of were produced from mice immunised with whole cells of heat-killed rough mutants of expressing partial or complete core LPS. MAbs were screened in an enzyme-linked immunosorbent assay (ELISA) against three different antigen cocktails: S-form LPS from three strains, R-form LPS from six strains and, as a negative control, R-form LPS from and . Selected MAbs were subsequently screened against a range of extracted LPS and whole cells from both reference strains and clinical isolates of . The antibodies were also screened in ELISA against whole-cell antigens from other spp. as well as strains of and . Five MAbs reacting with the core component of LPS were finally selected. Two of these, MAbs 360.7 and 304.1.4, were particularly reactive in immunoblots against unsubstituted core LPS, including that from O-antigenic serotypes of . The MAbs also reacted with some of the other spp., but not with or . Cross-reactivity with whole cells from other bacterial species was minimal or not observed. Reactivity of MAbs with some strains was observed, and binding to the protein A component was implicated. The reactivity of the MAbs was investigated further by flow cytometry and immunogold electronmicroscopy. The suitability of the MAbs for an immunological assay for detection of in respiratory secretions from CF patients is discussed.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1992
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1992-05-01
2024-04-16
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Production and characterisation of mouse monoclonal antibodies reactive with the lipopolysaccharide core of Pseudomonas aeruginosa
J. Med. Microbiol. 36, 358 (1992); https://doi.org/10.1099/00222615-36-5-358
/content/journal/jmm/10.1099/00222615-36-5-358
/content/journal/jmm/10.1099/00222615-36-5-358
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