Mechanisms of AllergyMatrix metalloproteinases and their inhibitors in the nasal mucosa of patients with perennial allergic rhinitis☆,☆☆
Section snippets
Subjects
Ten well-characterized subjects with perennial allergic rhinitis were recruited. All subjects had symptoms of perennial allergic rhinitis at the time of biopsy. Nasal examination demonstrated signs consistent with rhinitic mucosal inflammation, such as pale swollen mucosa, red engorged mucosa, and excess mucus. All of these rhinitic subjects had symptoms of bilateral or alternating nasal obstruction and rhinorrhea for at least 1 hour a day throughout the year. Skin prick tests were performed in
ELISA
Tissue was homogenized with lysis buffer. Concentrations of TIMP-1 and TIMP-2 were measured through use of commercially available Biotrak ELISA assay systems (Amersham Pharmacia Biotech, Amersham, United Kingdom) according to the manufacturer's instructions. Sufficient protein for assay was available from 8 rhinitic and 8 nonrhinitic subjects. Each assay was performed in duplicate. The optical density at 450 nm was read through use of a Titertek Multiscan Plus Elisa reader (EFLAB, Helsinki,
MMP and TIMP protein production in perennial allergic rhinitic and nonrhinitic nasal mucosa
Immunoreactive bands at the appropriate molecular weights for interstitial collagenase, gelatinase A, stromelysin-1, and gelatinase B were detected by means of Western blotting in 10 rhinitic and 10 nonrhinitic subjects. The latent forms of interstitial collagenase and stromelysin-1 in the rhinitic group were in general slightly higher than those of the nonrhinitic group, but low-molecular-weight active forms of enzymes were not detected. However, there was no difference in gelatinase A or
Discussion
In this study, we have demonstrated the presence of large amounts of mRNA for TIMP-1 and TIMP-2 and shown that the corresponding protein is abundant in both rhinitic and nonrhinitic subjects. Although MMP proteins were detected by Western blotting, mRNAs for interstitial collagenase, gelatinase A, stromelysin-1, and gelatinase B were not detected in large quantities in the majority of samples. We can conclude, therefore, that MMPs are not upregulated in the nasal mucosa in perennial allergic
Acknowledgements
We thank Dr G. Schultz, University of Florida, for the gift of MMP template (National Eye Institute Grant EY-05587).
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Nonasthmatic nasal polyposis patients with allergy exhibit greater epithelial MMP positivity
2009, Otolaryngology - Head and Neck SurgeryCitation Excerpt :Previously, several other reports mentioned that the expression level of TIMP-1 was significantly lower in nasal polyp patients compared to the chronic rhinosinusitis group.4,11 However, the study that compared the expression of TIMP-1 in NP and normal inferior turbinate mucosa showed no significant difference between those locations.8 Therefore, the difference of airway remodeling among the sites of nasal mucosa also should be further investigated in the future.
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Supported by the Special Trustees of the Royal London Hospitals NHS Trust, Department of Respiratory Medicine, The London Chest Hospital.
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Reprint requests: Sylvia L. F. Pender, PhD, Tissue Remodeling and Repair Group, Division of Infection, Inflammation and Repair, Mailpoint 813, Level E, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.