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Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion

Nature Medicine volume 2, pages 35–40 (1996)Cite this article

Abstract

Tissue factor is a transmembrane protein that activates the extrinsic coagulation pathway by binding factor VII. Endothelial cells, being in contact with circulating blood, do not normally express tissue factor. Here we provide evidence that oxygen free radicals induce tissue factor messenger RNA transcription and expression of tissue factor procoagulant activity in endothelial cells in culture. Isolated, perfused rabbit hearts exposed to exogenous oxygen free radicals also showed a marked increase in tissue factor activity within the coronary circulation. Furthermore, in ex vivo and in vivo hearts subjected to ischemia and reperfusion, a condition associated with a production of oxygen free radicals in large amounts, a marked increase in tissue factor activity occurred. This phenomenon could be abolished by oxygen radical scavengers. This increase in tissue factor activity during postischemic reperfusion was accompanied by a significant decrease in coronary flow, suggesting that increase in tissue factor activity with the consequent activation of the coagulation cascade might impair coronary flow during reperfusion and possibly contribute to the occurrence of reperfusion injury.

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Cardiology, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio, Nazionale dalle Ricerche, Second School of Medicine, University of Naples, Naples, Italy

    Paolo Golino, Massimo Ragni, Plinio Cirillo, Nicolino Esposito, Annalisa Scognamiglio, Bruno Trimarco, Guido Iaccarino, Mario Condorelli & Massimo Chiariello

  2. Department of Biology and Molecular and Cellular Pathology, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio, Nazionale dalle Ricerche, Second School of Medicine, University of Naples, Naples, Italy

    Antonio Feliciello

  3. Department of Experimental Medicine, School of Medicine at Catanzaro, University ofReggio Calabria, Italy

    V. Enrico Avvdimento

  4. Division of Cardiology, University of Perugia School of Medicine, Perugia, Italy

    Giuseppe Ambrosio

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  1. Paolo Golino
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Golino, P., Ragni, M., Cirillo, P. et al. Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion. Nat Med 2, 35–40 (1996). https://doi.org/10.1038/nm0196-35

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