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Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8

Abstract

RE-ESTABLISHING blood flow to ischaemic tissues causes greater injury than that induced during the ischaemic period1,2. This type of tissue injury, reperfusion injury, is involved in frostbite, multiple organ failure after hypovolaemia and in myocardial infarction1. Depletion of neutrophils alleviates reperfusion injury, implying a causal role of neutrophil infiltration3,4. Among members of the recently discovered family of chemotactic cytokines (chemo-kines)5–8, interleukin-8 (IL-8)5,9–13 is a major neutrophil chemotactic and activating factor produced by various types of human cells. We investigated its pathophysiological role in a rabbit model of a lung reperfusion injury. Reperfusion of ischaemic lung caused neutrophil infiltration and destruction of pulmonary structure, as well as local production of IL-8. Furthermore, the administration of a neutralizing monoclonal antibody against IL-8 prevented neutrophil infiltration and tissue injury, proving a causal role of locally produced IL-8 in this model.

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Sekido, N., Mukaida, N., Harada, A. et al. Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8. Nature 365, 654–657 (1993). https://doi.org/10.1038/365654a0

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