The Editors' choiceFunctional characterization of the atopy-associated gene PHF11
Section snippets
Methods
Additional methods for TH1 and TH2 cultures, cDNA synthesis and cloning, and immune precipitation assays and details of the antibodies used in this study appear in the Methods section of the Online Repository at www.jacionline.org.
Results
Using a well-characterized dataset of gene expression profiles from T cells, B cells, and other immune cells,9, 10 we found robust PHF11 expression in B- and T-cell subsets, including peripheral blood central (CCR7+CD4+CD45RO+) and effector (CCR7−CD4+CD45RO+) memory T cells. In the context of allergy, perhaps the most interesting result was the differential expression of PHF11 between TH1 and TH2 cells generated from neonatal CD4+ T cells, with the highest expression seen in TH1 cells (Fig 1, A
Discussion
In this article we show that (1) PHF11 expression is higher in TH1 than in TH2 cells; (2) siRNA knockdown of endogenous PHF11 or overexpression of PHF11 either decreased or increased the expression of TH1-type genes, respectively; (3) The G allele of the 3′UTR SNP rs1046295 that is preferentially transmitted to children with AD was associated with decreased PHF11 RNA in TH1 cells, and a predicted nonfunctional PHF11 isoform lacking exon II was more common in individuals carrying this G allele;
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2023, Chinese Medical Journal Pulmonary and Critical Care MedicineAllele-specific transcription of the asthma-associated PHD finger protein 11 gene (PHF11) modulated by octamer-binding transcription factor 1 (Oct-1)
2011, Journal of Allergy and Clinical ImmunologyCitation Excerpt :Studies of rs1046295 have shown the G allele to be associated with both increased IgE levels13 and asthma.39 Based on the results presented here and by Clarke et al,59 this may be a result of decreased expression and alternative splicing caused by the G allele because of differential binding of transcription factors. The chromosome 13q14 locus modulates total serum IgE levels, raised amounts of which are a main feature of atopic diseases such as asthma.
Supported by a grant from the National Health and Medical Research Council of Australia.
Disclosure of potential conflict of interest: M. S. Rolph has received research support from the National Health and Medical Research Council of Australia. G. J. Stewart has received research support from the National Health and Medical Research Council of Australia, the Australian Research Council, and Multiple Sclerosis Research Australia. G. J. Jones has received research support from the University of Sydney and the National Health and Medical Research Council of Australia. The rest of the authors have declared that they have no conflict of interest.