Rhinitis, sinusitis, and ocular diseasesProstaglandin, leukotriene, and lipoxin balance in chronic rhinosinusitis with and without nasal polyposis
Section snippets
Patients
Study subjects were selected on the basis of a documented medical history for CRS; a pathologic endoscopy, computed tomographic (CT) scan, or both; and the failure of 3 weeks of drug therapy (combined administration of antibiotics and oral glucocorticoids, 32-mg down to 8-mg daily dosage) to persistently relieve symptoms of this condition. Samples from ethmoidal and maxillary sinuses were collected from patients during functional endoscopic sinus surgery procedures in the Department of
mRNA expression by means of quantitative real-time PCR
Results of the quantitative real-time PCR are summarized in Fig 1. COX-2 expression was similar between the CRS and NM groups but was significantly downregulated in both nasal polyp groups (P = .0002), with a further decrease in the CRS-ASNP group compared with that seen in the CRS-NP group (P < .05). 15-LO mRNA expression was significantly increased in all patients with CRS compared with in healthy subjects (P = .0018). Interestingly, the CRS-ASNP group showed a downregulation of this enzyme
Discussion
The development of aspirin sensitivity remains unclear and has been related to several processes, including changes in AA metabolism, allergy, viral infection, and severity of inflammation.32, 33 We here show that COX-2 mRNA and PGE2 concentrations were significantly decreased in nasal polyp tissue, especially in patients with aspirin sensitivity (CRS-ASNP group). In contrast, the lowest levels of LTC4S, 5-LO mRNA, LTC4, LTD4, and LTE4 were observed in control subjects, and the highest levels
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Supported in part by grant BOF: 011D02903 given to Claudina A. Pérez-Novo by the University of Ghent.
Disclosure of potential conflict of interest: C. A. Pérez-Novo received a grant (BOF: 011D02903 [2003-2005]) from the University of Ghent, Belgium, which partially supported this work. P. Van Cauwenberge—none disclosed other than the grant received by C. A. Pérez-Novo.