Environmental and occupational respiratory disordersAcute LPS inhalation in healthy volunteers induces dendritic cell maturation in vivo
Section snippets
Healthy subjects undergoing LPS inhalation challenge
Nine healthy subjects (3 male, 6 female) between the ages of 18 and 50 years underwent LPS inhalation challenge. All subjects were nonsmoking volunteers with no history of lung disease (asthma, chronic obstructive pulmonary disease, fibrosis) and had been free of upper and lower respiratory tract infection for at least 4 weeks before beginning the study. All women provided a urine sample for pregnancy testing, and a positive pregnancy test resulted in exclusion from the study. All subjects had
Lung function, symptoms, vital signs, CBC analysis
Subjects did not develop systemic symptoms such as chills or cough, and no significant changes in vital signs (fever, blood pressure, heart rate, breath sounds) or lung function (% predicted forced vital capacity [FVC] and forced expiratory volume in 1 second [FEV1]) were observed at 1 hour, 6 hours, or 24 hours after CCRE (20,000 EU) challenge compared with before CCRE challenge. Peripheral blood CBC analysis showed no significant changes compared with pre-exposure baseline.
Neutrophil response
To examine whether
Discussion
In this study, we tested the hypothesis that stimulation of the lung innate immune system with inhaled LPS induces changes within cytokine and cellular milieu on the airways surface to generate and stimulate adaptive immune responses more effectively by promoting the maturation of lung DCs. We showed that inhaled clinical center reference endotoxin (CCRE, 20,000 EU) causes (1) a decrease in the phagocytic capacity of airways surface myeloid cells; (2) an increase in monocyte/macrophage
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Cited by (0)
Supported by National Institutes of Health grants RO1 HL62624 and RO1 HL66559 from the National Heart, Lung, and Blood Institute, P01AT002620 from the National Center for Complementary and Alternative Medicine, RR00046 from the General Clinical Research Centers program of the National Institutes of Health Division of Research Resources, and cooperative agreement CR-829522 from the US Environmental Protection Agency. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Heart, Lung, and Blood Institute, the National Center for Complementary and Alternative Medicine, the National Institutes of Health, or the US Environmental Protection Agency.