Asthma diagnosis and treatmentSevere asthma is associated with a loss of LX4, an endogenous anti-inflammatory compound
Section snippets
Patients
Subject characteristics are listed in Table I. Fifty-one subjects with asthma were selected according to the American Thoracic Society criteria.27 All had a reversible airway obstruction assessed by a 12% increase in FEV1 after inhalation of 200 μg salbutamol or a positive challenge with methacholine, with at least 20% fall in FEV1 and/or a 15% increase in FEV1 after a short course of oral glucocorticoids. The clinical severity of asthma was assessed according to GINA guidelines.28 Twelve
Patient characteristics
Demographic characteristics of all subjects are shown in Table I. Patients with severe asthma and COPD were older than patients with intermittent and moderate asthma and control subjects (P = .0002). Patients with intermittent asthma and COPD were not treated with steroids. FEV1 of all groups of subjects was significantly different (P < .001), and by using Bonferroni-Dunn posttest, significant differences were observed between all of the groups except patients with mild asthma versus controls and
Discussion
The current study demonstrates that patients with severe asthma present a persistent inflammation characterized by the proinflammatory IL-8 and LTB4 overproduction detected in supernatants of induced sputum. In the same way, we also observed that LTB4 was increased in supernatants of patients with COPD. Moreover, the anti-inflammatory LXA4 was present in supernatants of induced sputum and was increased in patients with mild asthma. Inversely, the anti-inflammatory LXA4 epimer, 15-epi-LXA4, was
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Supported by CIFRE research grant #99679 and by a grant from the Direction de la Recherche Clinique of CHU of Montpellier (AOI 1995 UF7515).