Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL-8, and tumor necrosis factor-α by peripheral mononuclear cells of workers with occupational asthma☆,☆☆,★
Section snippets
Reagents
RPMI 1640, HBSS, glutamine, penicillin-streptomycin, and HEPES were obtained from GIBCO Laboratories (Grand Island, NY); FBS was obtained from HyClone Laboratories, Inc (Logan, Utah); BSA (Fraction V, crystalline), alkaline phosphatase-goat anti-human IgG, alkaline phosphatase mouse monoclonal anti-rabbit IgG, and sodium pyruvate were obtained from Sigma Chemicals Co (St. Louis, Mo); goat anti-human IgE and alkaline phosphatase-rabbit anti-goat IgG were obtained from Kierkegard & Perry
Antigen enhancement of HRF and chemokine synthesis
Clinical characteristics of workers with DOA and antibody and cytokine responses are shown in Tables I and II.Empty Cell Empty Cell Exposure Empty Cell Empty Cell Empty Cell Empty Cell Empty Cell Empty Cell Empty Cell Worker nos. Chemical Duration (mos)* (mos) Cessation diagnosis Clinical challenge Bronchial AB‡ MCP-1§ IL-8§ TNF- α § Group 1: Symptomatic workers 1 MDI, TDI 48 19 OA +(lab)∥, LAR¶ IgG + + – 2 MDI 25 10 OA +(work)# – + + + 3 HDI, MDI 100 32 OA +(work) – + + + 4 HDI 60 1 OA +(work) IgE, IgG – + + 5 HDI 240 19 OA +(work) – + + – 6 HDI 12 11 OA +(work) IgE, IgG + + + 7 HDI, MDI 60 20 OA +(work)
DISCUSSION
In this study workers with confirmed DOA exhibited enhanced in vitro diisocyanate-HSA antigen-stimulated synthesis of MCP-1, IL-8, and TNF-α. Diisocyanate-antigen enhancement of MCP-1 and IL-8 was demonstrated in 7 of 8 workers with confirmed OA. Of these subjects, 6 had been completely restricted from exposure to diisocyanate chemicals for longer than 10 months, indicating persistence of diisocyanate-responsive mononuclear cells. Diisocyanate-antigen specificity of MCP-1 production was
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Cited by (0)
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From athe Department of Internal Medicine, Immunology Division, University of Cincinnati College of Medicine, Cincinnati; and bthe Department of Internal Medicine, Allergy Division, University of Texas Medical Branch, Galveston.
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Reprint requests: Zana L. Lummus, University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Immunology, 231 Bethesda Avenue, Cincinnati, OH 45267-0563.
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