Diurnal variation of urinary leukotriene E4 and histamine excretion rates in normal subjects and patients with mild-to-moderate asthma☆,☆☆,★
Section snippets
Subjects
Five normal male volunteers (38 to 54 years of age) and eight patients with stable mild-to-moderate asthma (4 men and 4 women; 19 to 50 years of age) were enrolled in this study. None of the normal subjects had a history of atopic or airway disease; none were taking any medication. All patients (7 atopic, 1 nonatopic [subject D]) met the American Thoracic Society criteria for the diagnosis of asthma11 and were receiving treatment with β-agonists alone. They were free of asthma exacerbation for
RESULTS
All patients with asthma remained in stable condition during the study while using only inhaled β-agonists. FEV1 values for each subject are given in Table I. There were no significant differences in FEV1 measured at 8:00 AM compared with that measured at 4:00 PM, during the course of the study in either the normal or the asthma group. All subjects had normal red cell, white blood cell, and platelet counts and normal serum chemistry values.
DISCUSSION
The recovery of inflammatory mediators from the urine has the potential to provide a rapid, safe, and easily available method of estimating mediator synthesis and release. However, because it is well established that many endogenous biomolecules are not excreted constantly over the course of a 24-hour period,8 it is important to determine whether a given mediator is excreted at a constant rate. In this study we examined urinary excretion of two mediators of bronchoconstriction, LTE4 and
Acknowledgements
We thank Juan Du for her excellent technical assistance with the LTE4 assay. Data organization and analysis were performed on the PROPHET system, a national computer resource sponsored by the National Center for Research Resources, National Institutes of Health.
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From aCombined Program in Pulmonary and Critical Care Medicine and bCharles A. Dana Research Institute and the Harvard-Thorndike Laboratory of Beth Israel Hospital, Departments of Medicine, Beth Israel Hospital, Brigham and Women’s Hospital and Harvard Medical School.
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Reprint requests: Jeffrey M. Drazen, MD, Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 75 Francis St., Boston, MA 02115.
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