Asthma, Rhinitis, Other Respiratory DiseasesIncreased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatic subjects with sputum eosinophilia☆
Section snippets
Patients
The study included 14 control subjects and 30 patients with asthma. Control subjects (age median, 31; 25th to 75th percentiles, and 29 to 37 years) never had asthma, allergic diseases, or chronic bronchitis. All the subjects were lifelong nonsmokers, and their pulmonary functions were within the normal range.
Asthmatic subjects (median age, 38; 29 to 55 years) were diagnosed on the basis of criteria previously described in detail,11 and they had objective evidence of variable airflow obstruction
Study subjects
The median age of patients with asthma and of control subjects was not statistically different. FEV1 values of control subjects had a median of 101% of the predicted value (25th to 75th percentiles, 100 to 105). The median of FEV1 values for the asthmatic subjects resulted of 81% (72 to 94; P < .001). Fifteen untreated asthmatic subjects had a FEV1 value of <80%. Atopy was present in 11 asthmatic subjects (Table I).
Total and differential cell counts in induced sputum samples
The median volume of recovered supernatants resulted of 4.7 mL (3 to 8.4) in
Discussion
The results of this study showed that PGE2 concentrations in induced sputum samples of asthmatic subjects directly correlate with eosinophilic inflammation markers such as the concentration of ECP or the number of eosinophils present, pointing out a potential link between AA cyclooxygenase metabolites and eosinophilic infiltration and inflammation.
Recently, a subdivision of asthma phenotype based on the presence of high or low eosinophil infiltrate has been proposed21 suggesting that in
Acknowledgements
SC-58236 was kindly provided by Dr Peter Isakson, Searle, St Louis, Mo.
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Supported in part by the University of Milan, FIRST (A.S.).