[59] Glutathione disulfide (GSSG) efflux from cells and tissues

doi:10.1016/S0076-6879(84)05062-X

Methods in Enzymology

Volume 105, 1984, Pages 445-451

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Because of the release of glutathione disulfide (GSSG) into the extracellular space, cells, such as erythrocytes, or tissues, such as eye lens or liver, were found to respond to oxidizing conditions. Under steady-state conditions, the rate of metabolism of an externally added model hydroperoxide, t-butyl hydroperoxide, was observed to be linearly related to the rate of efflux of GSSG from the isolated perfused rat liver; in this organ, about 3% of the flux through glutathione (GSH) peroxidase is represented by an extracellular appearance of GSSG. Thus, even though most cell types contain an appreciable activity of GSSG reductase, a rise in the intracellular production of GSSG is accompanied by an export of the disulfide; apparently, there are multiple ways of disposing of GSSG, which may accumulate. Based on the relation between hydroperoxide metabolism and GSSG efflux, it was proposed that the latter might be a useful extracellular parameter to assess hydroperoxide turnover in cells and tissues. Because of the multiple factors that may influence the rate of GSSG efflux, appropriate caution is required in each particular case in which the parameter is used.

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[59] Glutathione disulfide (GSSG) efflux from cells and tissues

Publisher Summary

J. Biol. Chem.

FEBS Lett.

Anal. Biochem.

Anal. Biochem.

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Anal. Biochem.

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J. Biol. Chem.

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J. Nutr.

Biochim. Biophys. Acta