The α and π isoenzymes of glutathione S-transferase in human fetal lung: in utero ontogeny compared with differentiation in lung organ culture

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Abstract

Polyclonal antisera to the α and π isoenzymes of glutathione S-transferase have been used in immunohistochemical studies of developing human lung. In utero expression of the π set was down-regulated in distal airway cells and the first appearance of π-negative cells coincided with phenotypic differentiation. In contrast, in the early phase of fetal lung organ culture π isoenzyme was detected in all differentiated epithelial cells and only as culture progressed did focal negativity develop. The α set showed no developmental changes in utero or in organ culture.

Reference (22)

  • HirrellP.A. et al.

    Biochim. Biophys. Acta

    (1987)
  • FryerA.A. et al.

    Biochim. Biophys. Acta

    (1986)
  • SorokinS.

    Develop. Biol.

    (1961)
  • InselmanL.S. et al.

    J. Pediatr.

    (1981)
  • StrangeR.C. et al.

    Biochim. Biophys. Acta

    (1988)
  • MannervikB. et al.
  • StockmanP.K. et al.

    Biochem. J.

    (1985)
  • BoardP.G. et al.
  • StrangeR.C. et al.

    Biochem. Genet.

    (1985)
  • PacificiG.M. et al.

    Biochem. Pharmacol.

    (1985)
  • GuthenbergC. et al.

    Biochem. J.

    (1986)

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