[241 Total antioxidant status in plasma and body fluids

doi:10.1016/0076-6879(94)34095-1

Methods in Enzymology

Volume 234, 1994, Pages 279-293

https://doi.org/10.1016/0076-6879(94)34095-1 Get rights and content

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This chapter discusses the total antioxidant status in plasma and body fluids. Methods that have been developed for the measurement of the antioxidant activity of fluids are all essentially inhibition methods in which a free radical species is generated, there is an end point by which the presence of the radical is detected, and the antioxidant activity of the added sample inhibits the end point by scavenging the free radical. Methods vary greatly as to the radical that is generated, the reproducibility of the generation process, and the end point that is used. Currently, the telomeric repeat amplification protocol (TRAP) assay is the most widely used assay of antioxidant activity. Other methods are based on inhibition of spontaneous tissue autoxidation by antioxidants. Thermal decomposition of the water-soluble azo compound 2,2'-azobis(2-amidopropane) hydrochloride (ABAP) yields peroxyl radicals at a known and constant rate. Each molecule of Trolox, α-tocopherol, or other phenolic antioxidant traps two peroxyl radicals, giving Trolox a stoichiometric factor of 2.0 in the TRAP assay. Other pure antioxidants have different stoichiometric factors (ascorbate, 1.5; urate, 1.7), and these must be taken into account when extrapolating back to molar concentrations from TRAP values.

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[241 Total antioxidant status in plasma and body fluids

Publisher Summary

FEBS Lett.

Arch. Biochem. Biophys.

Anal. Biochem.

Anal. Biochem.

Clin. Chim. Acta

Anal. Chim. Acta

J. Biol. Chem.