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Genomovar Diversity Amongst Burkholderia cepacia Complex Isolates From an Australian Adult Cystic Fibrosis Unit

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Abstract

In this study, a combination of recA-based PCR assays and 16S rDNA restriction fragment length polymorphism (RFLP) analysis was used to determine the genomovar diversity of clinical Burkholderia cepacia complex isolates. Twenty-eight isolates were prospectively collected from patients attending a large Australian adult cystic fibrosis (CF) unit, 22 isolates were referred from other Australian CF units and a further eight isolates originated from patients without CF. The 28 prospectively collected isolates were distributed amongst the following genomovars: Burkholderia cepacia genomovar I (28.6%), Burkholderia multivorans (21.4%), Burkholderia cepacia genomovar III (39.3%), Burkholderia vietnamiensis (3.6%) and Burkholderia ambifaria (7.1%). The results of this study highlight the usefulness of 16S rDNA RFLP typing for the identification of other Burkholderia spp. and non-fermenting gram-negative bacteria.

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Acknowledgements

This study was supported by The Prince Charles Hospital Foundation and the Trust Fund of the Queensland Health Pathology Service. We gratefully acknowledge Dr. Philip Masel for allowing us the opportunity to study patients under his care, the Staff of the Microbiology Department and clinicians and scientists who kindly referred isolates from the following centres: The Royal Brisbane and Royal Childrens Hospitals, Sullivan Nicolaides Pathology, Queensland Medical Laboratory, Mater Misericordiae Hospital, Brisbane; Royal Prince Alfred Hospital, Westmead Hospital, Sydney; The Alfred Hospital, Melbourne; Institute for Medical and Veterinary Science, and the Women's and Childrens Hospital, Adelaide. We also thank Dr. J.E. Moore, for his kind provision of a number of Burkholderia cepacia control strains. The experiments performed in this study comply with current Australian law.

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Authors and Affiliations

  1. Department of Microbiology, Queensland Health Pathology Service, The Prince Charles Hospital, Rode Road, Chermside, Brisbane, 4032 Queensland, Australia

    T. J. Kidd

  2. Adult Cystic Fibrosis Unit and Department of Medicine, University of Queensland, The Prince Charles Hospital, Brisbane, Queensland, Australia

    S. C. Bell

  3. Department of Microbiology and Infectious Diseases Unit, The Prince Charles Hospital, Brisbane, Queensland, Australia

    C. Coulter

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  1. T. J. Kidd
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  2. S. C. Bell
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  3. C. Coulter
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Correspondence to T. J. Kidd.

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Kidd, T.J., Bell, S.C. & Coulter, C. Genomovar Diversity Amongst Burkholderia cepacia Complex Isolates From an Australian Adult Cystic Fibrosis Unit. Eur J Clin Microbiol Infect Dis 22, 434–437 (2003). https://doi.org/10.1007/s10096-003-0949-8

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