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Transcriptional regulation of the acetyl-CoA synthetase gene acsA in Pseudomonas aeruginosa

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Abstract

Pseudomonas aeruginosa ATCC 17933 is able to oxidize ethanol to acetate under aerobic conditions. The P. aeruginosa acetyl-CoA synthetase (ACS) gene acsA was previously identified, and the ACS enzyme described to be required for growth on ethanol as the sole source of carbon and energy. Here, we investigated the transcriptional regulation of the acsA gene using an acsA::lacZ fusion. Transcription of acsA was regulated by the carbon source, and expression was maximal on ethanol, acetate and propionate. In addition, the induction depended on the response regulator ErdR, which also regulates hierarchically arranged genes for ethanol oxidation. Transcription of the acsA gene was repressed by addition of succinate to an ethanol-containing medium. This repression required Crc, the product of the catabolite repression control gene crc.

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Fig. 1

Abbreviations

ACS:

Acetyl-CoA synthetase

crc:

Catabolite repression control

ACK:

Acetate kinase

PTA:

Phosphotransacetylase

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Authors and Affiliations

  1. Fachgebiet Angewandte Biochemie, Institut für Biotechnologie, Technische Universität Berlin, Seestraße 13, 13353, Berlin, Germany

    Utta Kretzschmar, Viola Khodaverdi & Lorenz Adrian

  2. Helmholtzzentrum für Umweltforschung (UFZ), Permoser Straße 15, 04318, Leipzig, Germany

    Lorenz Adrian

Authors
  1. Utta Kretzschmar
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  2. Viola Khodaverdi
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  3. Lorenz Adrian
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Correspondence to Lorenz Adrian.

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Communicated by Timothy Donohue.

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Kretzschmar, U., Khodaverdi, V. & Adrian, L. Transcriptional regulation of the acetyl-CoA synthetase gene acsA in Pseudomonas aeruginosa . Arch Microbiol 192, 685–690 (2010). https://doi.org/10.1007/s00203-010-0593-5

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  • DOI: https://doi.org/10.1007/s00203-010-0593-5

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