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Peripheral blood neutrophil production of interleukin-1 receptor antagonist and interleukin-1β

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Abstract

The objective of this study was to characterize interleukin-1 receptor antagonist (IL-1ra) and interleukin-1β (IL-1β) production by human peripheral blood neutrophils (polymorphonuclear leukocytes; PMN). Unstimulated PMN contained IL-1ra protein in the absence of IL-1ra mRNA; IL-1β mRNA and protein were undetectable. Lipopolysaccharide (LPS), granulocyte/macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-α), individually, transiently increased IL-1ra steady-state mRNA levels in PMN, with associated increases in IL-1ra protein synthesis. LPS, GM-CSF, and TNF-α generated similar increases in IL-1β mRNA, yet only LPS resulted in detectable synthesis of IL-1β protein. IL-4 enhanced LPS-induced IL-1ra production by PMN and inhibited LPS-induced IL-1β production. IL-1ra protein present within stimulated PMN supernatants existed in the 22- to 25-kD glycosylated form. Polymerase chain reaction amplification determined that only sIL-1ra mRNA was present within stimulated PMN; icIL-1ra mRNA was undetectable. These results indicate that freshly isolated PMN possess a small amount of IL-1ra protein and that these cells can respond to stimuli with a low level of sIL-1ra transcription and translation. PMN may be a major source of IL-1ra in inflammatory exudates where these cells predominate.

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Authors and Affiliations

  1. Division of Rheumatology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box B-115, 80262, Denver, Colorado

    Mark Malyak, Michael F. Smith Jr., Ashley A. Abel & William P. Arend

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  1. Mark Malyak
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  2. Michael F. Smith Jr.
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  3. Ashley A. Abel
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  4. William P. Arend
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Malyak, M., Smith, M.F., Abel, A.A. et al. Peripheral blood neutrophil production of interleukin-1 receptor antagonist and interleukin-1β. J Clin Immunol 14, 20–30 (1994). https://doi.org/10.1007/BF01541172

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  • DOI: https://doi.org/10.1007/BF01541172

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