Elsevier

Genomics

Volume 45, Issue 1, 1 October 1997, Pages 17-23
Genomics

Regular Article
Molecular Characterization and Pattern of Tissue Expression of the Gene for Neutrophil Gelatinase-Associated Lipocalin from Humans,☆☆

https://doi.org/10.1006/geno.1997.4896Get rights and content

Abstract

Neutrophil gelatinase-associated lipocalin (NGAL) is a 25-kDa lipocalin first identified as a protein stored in specific granules of the human neutrophil. The protein is believed to bind small lipophilic substances such as bacterial derived formylpeptides and lipopolysaccharides (LPS) and might function as a modulator of inflammation. To characterize the regulation of NGAL further, we have cloned and sequenced a 5869-bp region of the NGAL gene including 1695 bp of the 5′ nontranscribed region and a 3696-bp coding region encompassing seven exons and six introns. The transcriptional start sites were identified by an RNase protection assay. The NGAL gene is highly homologous to the mouse gene 24p3. NGAL was expressed in bone marrow and in tissues that are prone to exposure to microorganisms. Potentialcis-acting elements were identified in the promoter region of the NGAL gene by computer analysis and include binding sites for CTF/CBP, the hematopoietic transcription factors GATA-1 and PU.1, and the LPS-inducible factor NF-κB.

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The HGMW-approved symbol for the gene described in this paper is LCN2.

☆☆

Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession No. X99133.

1

To whom correspondence should be addressed at Rigshospitalet L-4041, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark. Telephone: +45 35 45 48 86. Fax: +45 35 45 48 41. E-mail: [email protected].

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