Summary table of experiments and statistical tests including samples number, sample type, associated figure code, statistical test and summary p values generated by analysis of variance (ANOVA) or unpaired t-tests
| Sample type | Experiment | Figure code | P value | Statistical test | Multiple comparison |
| Cell line | CFBE versus HBE (n=3) NTA | 1B(i) | <0.0001 | Unpaired t-test | None |
| Cell line | CFBE versus HBE (n=3) NTA | 1B(ii) | <0.0001 | Unpaired t-test | None |
| Cell line | Cufi-5 versus Nuli-1 (n=3) NTA | 1E(i) | 0.2504 | Unpaired t-test | None |
| Cell line | Cufi-5 versus Nuli-1 (n=3) NTA | 1E(ii) | 0.0209 | Unpaired t-test | None |
| BALF | PWCF (n=3)–4 age groups NTA | 1G(i) | <0.0001 | One-way ANOVA | Bonferroni |
| BALF | PWCF (n=3)–4 age groups NTA | 1G(ii) | <0.0001 | One-way ANOVA | Bonferroni |
| BALF | PWCF (n=12) versus controls NTA | 1I(i) | <0.0001 | One-way ANOVA | Bonferroni |
| BALF | PWCF (n=12) versus controls NTA | 1I(ii) | <0.0001 | One-way ANOVA | Bonferroni |
| BALF | PWCF (n=6) adults versus controls NTA | 1J | 0.0002 | Unpaired t-test | None |
| Cell line | CFBE versus HBE autophagy stimulators (n=3) NTA | 2B | 0.0002 | One-way ANOVA | Bonferroni |
| Cell line | CuFi-5 versus NuLi-1 autophagy stimulators (n=3) NTA | 2D | <0.0001 | One-way ANOVA | Bonferroni |
| Cell line | CFBE versus HBE correctors (n=3) NTA | 2F | <0.0001 | One-way ANOVA | Bonferroni |
| Cell line | CuFi-5 versus NuLi-1 correctors (n=3) NTA | 2H | 0.0002 | One-way ANOVA | Bonferroni |
| Cell line | CuFi-4 versus NuLi-1 potentiator (n=3) NTA | 2I(i) | <0.0001 | Unpaired t-test | None |
| Cell line | CuFi-4 versus NuLi-1 potentiator (n=3) NTA | 2I(ii) | 0.0036 | Unpaired t-test | None |
| BALF | PWCF (n=4) pre/post ivacaftor | 2J(i) | <0.0001 | Unpaired t-test | None |
| BALF | PWCF (n=4) pre/post ivacaftor | 2J(ii) | 0.3689 | Unpaired t-test | None |
| BALF | PWCF (n=20), control (n=10), proteomics | 3G | <0.0001 | Hypergeometric test/Bonferroni | None |
| BALF | PWCF (n=20), control (n=10), proteomics | 4A | <0.0001–0.0453* | Unpaired t-test | None |
| BALF | PWCF (n=20), control (n=10), proteomics | 4B | 0.0040–0.0346* | One-way ANOVA | Bonferroni |
| BALF | PWCF (n=20), control (n=10), proteomics | 4C | 0.0006–0.0494* | Pearson Correlation | None |
| Serum | Healthy controls (n=3), flow cyometry | 5B | 0.0497 | Unpaired t-test | None |
| Serum | Healthy controls (n=3), MPO activity | 5C | 0.0153 | One-way ANOVA | Bonferroni |
| Serum | Healthy controls (n=4), neutrophil migration | 5D | 0.0352 | One-way ANOVA | Bonferroni |
| Serum | Healthy controls (n=3), neutrophil migration | 5F | 0.0159 | One-way ANOVA | Bonferroni |
| Serum | PWCF (n=8), controls (n=4), neutrophil migration | 5G | 0.0024 | Two-way ANOVA | Bonferroni |
| Serum | PWCF (n=3) neutrophil migration | 5H | 0.0014 | One-way ANOVA | Bonferroni |
*P value range is provided as multiple proteins analysed.
BALF, bronchoalveolar lavage fluid; MPO, myeloperoxidase; NTA, nanoparticle tracking analysis; PWCF, people with CF.