Conditions during ex-vivo ECMO
| 40×106 iPSC-MSCs (n=4) | 20×106 iPSC-MSCs* (n=4) | Control (n=6) | |
| Time to 25% decrease in blood flow (min±SE) | 68±32 | 99±21 | – |
| Time to 50% decrease in blood flow (min±SE) | 85±39 | – | – |
| Blood flow at 2000 RPM (L/min±SE) | |||
| 30 s | 4.05±0.02 | 4.04±0.02 | 4.03±0.05 |
| 15 min | 3.79±0.35 | 4.01±0.04 | 4.03±0.05 |
| 30 min | 2.83±0.98 | 4.12±0.07 | 3.98±0.06 |
| 60 min | 1.94±1.14 | 3.95±0.20 | 4.12±0.03 |
| 120 min | 1.39±0.92 | 4.09±0.05 | |
| 240 min | 0.60±0.35 | 4.12±0.03 | |
| Transoxygenator pressure gradient (mm Hg±SE) | |||
| 30 s | 20±6 | 21±2 | 19±3 |
| 15 min | 27±12 | 21±3 | 21±2 |
| 30 min | 51±24 | 22±3 | 25±4 |
| 60 min | 55±31 | 28±4 | 24±3 |
| 120 min | 50±18 | 25±4 | |
| 240 min | 101±9 | 21±4 | |
| MSCs detectable in blood (cells/µL±SE) | |||
| 30 s | 21.8±1.9 | ||
| 30 min | 9.4±4.2 | ||
| 60 min | 6.9±3.4 | ||
| 120 min | 4.1±3.8 | ||
| 240 min | 0.3±0.2 | ||
↵*Circuits terminated after a 25% reduction in blood flow (3 L/min), performed to optimise conditions for microscopy.
–, Did not occur; ECMO, extracorporeal membrane oxygenation; iPSC, induced pluripotent stem cell; MSCs, mesenchymal stem cells; RPM, revolutions per minute.