Inhibition of NOTCH3 abrogates of RV-induced goblet cell metaplasia in COPD cell cultures
| RV-induced changes | % inhibition | ||
| NT shRNA | NOTCH1 shRNA | NOTCH3 shRNA | |
| Goblet cells | 0 | 0.00±51.22 | 79.87±28.72 |
| FOXA3 | 0 | 0.00±62.07 | 90.17±3.96 |
| MUC5B | 0 | 2.75±11.58 | 79.55±4.12 |
| MUC5AC | 0 | 0.00±35.52 | 75.82±8.32 |
| HEY-1 | 0 | 0.00±36.11 | 77.10±7.87 |
COPD cells transfected with NT, NOTCH1 or NOTCH3 were infected with RV or sham and incubated for 15 days. Number of goblet cells and mRNA levels of FOXA3, MUC5B, MUC5AC and HEY1 was determined, fold increase for RV over respective sham was calculated for each parameter and % inhibition was assessed by comparing to NT shRNA-transfected group.
COPD, chronic obstructive pulmonary disease; NT, non-targeting; RV, rhinovirus.