Inter-batch variance. (A) Inter-batch variation between ECLs obtained from American e-liquids store was assessed by GC-FID to determine nicotine content. (B) Inter-batch variation between preparations of condensate was assessed by measurement of physical characteristics, including volume recovered, nicotine recovery, optical density and cytotoxic potential following a 24 hour challenge with each condensate. Preparations 1–3 were produced from ECL batch 1, preparations 4–6 were produced from ECL batch 2
| (A) | ECL batch | |
| 1 | 2 | |
| Expected nicotine (mg/mL) | 36.00 | 36.00 |
| Observed nicotine (mg/mL) | 31.00 | 30.70 |
| Disparity | 13.89 | 14.72 |
| Mean OD 370 nm | 0.22 | 0.21 |