List of genes differentially expressed by hypercapnia in severe injury VILI
| Expression change by microarray | Expression by RTPCR (relative to NV; mean±SEM) | ||||
|---|---|---|---|---|---|
| Symbol | Gene | p Value* (hyper vs normo) | Fold change (hyper vs normo) | Normo | Hyper |
| Ttpa | α-tocopherol transfer protein | 7.99E-07 | 1.74 | 0.94±0.12 | 2.11±0.42† |
| Tat | tyrosine aminotransferase | 8.56E-04 | 1.71 | not tested | |
| Pmvk | phosphomevalonate kinase | 3.80E-05 | 1.66 | 4.70±0.70 | 8.50±1.20‡ |
| P2ry10 | purinergic receptor P2Y, G-protein coupled 10 | 2.33E-03 | 1.55 | 1.60±0.16 | 3.04±0.88 |
| Fat3 | FAT tumour suppressor homologue 3 (Drosophila) | 5.31E-05 | 1.53 | not tested | |
| Zc3h6 | zinc finger CCCH type containing 6 | 1.56E-05 | 1.53 | not tested | |
| Cckar | cholecystokinin A receptor | 2.78E-04 | 1.52 | 0.32±0.03 | 0.48±0.06 |
| Plcxd3 | phosphatidylinositol-specific phospholipase C, X domain c | 3.35E-08 | 1.50 | not tested | |
| Gsta2 | glutathione S-transferase, α 2 (Yc2) | 1.30E-05 | −1.50 | not tested | |
| Ctps | cytidine 5’-triphosphate synthase | 3.89E-07 | −1.52 | not tested | |
| Pus7l | pseudouridylate synthase 7 homologue (S. cerevisiae)-like | 3.67E-06 | −1.52 | not tested | |
| Pdgfb | platelet-derived growth factor, B polypeptide | 8.12E-06 | −1.56 | 1.08±0.10 | 0.55±0.06† |
| Scel | sciellin | 1.16E-03 | −1.58 | not tested | |
| Sh2d4a | SH2 domain containing 4A | 1.80E-09 | −1.58 | not tested | |
| Ereg | epiregulin | 8.22E-07 | −1.59 | 5.82±0.95 | 2.27±0.27‡ |
| Egr2 | early growth response 2 | 1.43E-04 | −1.60 | 9.21±1.80 | 4.23±0.57‡ |
| Tnc | tenascin C | 2.94E-04 | −1.61 | 4.21±1.01 | 1.60±0.28‡ |
| Gsta1 | glutathione S-transferase, α 1 (Ya) | 1.71E-05 | −1.62 | 6.91±1.33 | 2.58±0.45† |
| Rnd1 | Rho family GTPase 1 | 1.74E-04 | −1.63 | not tested | |
| Mthfd2 | methylenetetrahydrofolate dehydrogenase | 2.44E-05 | −1.64 | not tested | |
| Ccl7 | chemokine (C-C motif) ligand 7 | 1.26E-06 | −1.65 | not tested | |
| Ctgf | connective tissue growth factor | 1.39E-04 | −1.67 | 3.95±0.822 | 1.95±0.42 |
| Slc26a4 | solute carrier family 26, member 4 | 9.52E-06 | −1.75 | not tested | |
| Timp1 | tissue inhibitor of metalloproteinase 1 | 5.00E-05 | −1.82 | not tested | |
| Ncr1 | natural cytotoxicity triggering receptor 1 | 5.82E-07 | −1.82 | 0.65±0.07 | 0.26±0.03† |
| Cxcl5 | chemokine (C-X-C motif) ligand 5 | 7.43E-05 | −1.88 | not tested | |
| Spic | Spi-C transcription factor (Spi-1/PU.1 related) | 1.07E-03 | −1.96 | not tested | |
| Hmox1 | heme oxygenase (decycling) 1 | 6.72E-05 | −1.98 | 12.9±1.99 | 4.51±0.59 |
| Ccl2 | chemokine (C-C motif) ligand 2 | 2.16E-06 | −2.05 | not tested | |
*Adjusted p value, FDR <0.05, Benjamini Hochberg.
†p<0.01 vs high stretch normocapnia; N=10/group.
‡p<0.05 vs high stretch normocapnia.