Staining of lung biopsy specimens
| Stain | Purpose |
|---|---|
| Always: H&E | Overview of extent, distribution and nature of any pathology |
| Always: Elastic Van Gieson | Collagen/pulmonary vasculature |
| As indicated: CD34 | Vascular marker |
| As indicated: Perls stain | Iron-laden macrophages |
| As indicated: Bombesin | Diagnosis of NEHI |
| As indicated: Periodic Schiff | Glycogen-positive cells: PIG |
| If infection suspected | Ziehl–Neelsen, Grocott, others |
| As indicated: immunohistochemistry | Langerhans cells (S-100, CD1a) |
NEHI, neuroendocrine cell hyperplasia of infancy.