Table 3

Staining of lung biopsy specimens

StainPurpose
Always: H&EOverview of extent, distribution and nature of any pathology
Always: Elastic Van GiesonCollagen/pulmonary vasculature
As indicated: CD34Vascular marker
As indicated: Perls stainIron-laden macrophages
As indicated: BombesinDiagnosis of NEHI
As indicated: Periodic SchiffGlycogen-positive cells: PIG
If infection suspectedZiehl–Neelsen, Grocott, others
As indicated: immunohistochemistryLangerhans cells (S-100, CD1a)
  • NEHI, neuroendocrine cell hyperplasia of infancy.