Components of the non-neuronal cholinergic system expressed in epithelial cells
| 16HBE14o− cells | Submerged cultured HAE cells | Freshly isolated HAE cells | ALI-cultured HAE cells | |
|---|---|---|---|---|
| CHT1 | 27.7 (±0.5) | 24.5 (±1.3) | 17.1 (±0.4) | 24.9 (±0.7) |
| CTL1 | 10.6 (±0.3) | 12.9 (±0.6) | 14.3 (±0.6) | 11.7 (±0.5) |
| CTL4 | 23.5 (±0.4) | 17.5 (±0.9) | 11.9 (±0.6) | 10.3 (±0.7) |
| CarAT | 15.5 (±1.5) | 14.7 (±0.8) | 14.2 (±0.7) | 15.3 (±0.8) |
| AChE | 22.4 (±1.0) | 17.8 (±1.1) | 18.5 (±1.3) | 21.9 (±0.8) |
| BChE | 19.7 (±0.8) | 16.5 (±0.6) | 18.9 (±1.3) | 19.4 (±0.6) |
| M1R | 18.7 (±1.4) | 17.8 (±0.7) | 17.1 (±1.0) | 21.2 (±0.2) |
| M2R | 21.5 (±0.5) | 19.3 (±0.8) | 17.5 (±0.8) | 21.1 (±0.4) |
| M3R | 25.9 (±0.9) | 19.8 (±1.0) | 18.7 (±1.4) | 17.6 (±0.6) |
Expression of the components was analysed by real-time quantitative PCR in submerged 16HBE14o− cells and submerged HAE cell cultures, in freshly isolated HAE cells, and in ALI-cultured HAE cells. Data represent mean (±SEM) Ct values corrected for 18S (ie, highest values represent lowest expression levels), n=3.
AChE, acetylcholinesterase; BChE, butyrylcholinesterase; CarAT, carnitine acetyltransferase; CHT1, high-affinity choline transporter 1; CTL1, choline transporter-like protein 1; CTL4, choline transporter-like protein 4; HAE, human airway epithelial; M1R, muscarinic M1 receptor; M2R, muscarinic M2 receptor; M3R, muscarinic M3 receptor.