Predictors of the severity of different histological features on univariable and multivariable linear regression analysis in the whole NAFLD population (n=80)
| Univariable | Multivariable | |||||
|---|---|---|---|---|---|---|
| β | SE (β) | p Value | β | SE (β) | p Value | |
| Predictors of steatosis score | ||||||
| SaO2 <90%(%TST) | 0.593 | 0.113 | <0.001 | 0.339 | 0.101 | <0.001 |
| TLR-4-positive hepatocytes (%) | 0.601 | 0.123 | <0.001 | 0.345 | 0.127 | 0.008 |
| L/M ratio | 0.235 | 0.139 | 0.042 | – | – | – |
| Plasma adiponectin | −0.348 | 0.120 | 0.011 | – | – | – |
| Plasma LPS | 0.426 | 0.169 | 0.009 | – | – | – |
| AHI | 0.434 | 0.131 | 0.010 | – | – | – |
| Nadir SaO2 (%) | −0.228 | 0.117 | 0.032 | – | – | – |
| Adiponectin-positive hepatocytes(%) | −0.346 | 0.139 | 0.019 | – | – | – |
| Predictors of hepatocyte ballooning | ||||||
| Plasma LPS | 0.611 | 0.109 | <0.001 | 0.341 | 0.113 | 0.009 |
| SaO2 <90% (%TST) | 0.682 | 0.141 | <0.001 | 0.364 | 0.145 | 0.002 |
| TLR-4-positive hepatocytes (%) | 0.694 | 0.173 | <0.001 | 0.449 | 0.124 | <0.001 |
| Hepatocyte apoptotic index | 0.298 | 0.112 | 0.039 | – | – | – |
| TLR-4-positive Kupffer cells (n) | 0.312 | 0.179 | 0.031 | – | – | – |
| Predictors of lobular inflammation | ||||||
| Plasma LPS | 0.587 | 0.138 | <0.001 | 0.438 | 0.122 | 0.001 |
| TLR-4-positive Kupffer cells (n) | 0.501 | 0.141 | <0.001 | 0.396 | 0.118 | 0.011 |
| BMI z-score | 0.312 | 0.114 | 0.030 | – | – | – |
| Plasma adiponectin | −0.422 | 0.189 | 0.009 | – | – | – |
| AHI | 0.274 | 0.109 | 0.028 | – | – | – |
| SaO2 <90% (%TST) | 0.270 | 0.134 | 0.031 | – | – | – |
| Predictors of NAS | ||||||
| SaO2 <90% (%TST) | 0.512 | 0.102 | <0.001 | 0.343 | 0.102 | 0.011 |
| Plasma LPS | 0.632 | 0.121 | <0.001 | 0.429 | 0.076 | 0.001 |
| TLR-4-positive hepatocytes (%) | 0.551 | 0.169 | <0.001 | 0.352 | 0.089 | 0.009 |
| TLR-4-positive Kupffer cells (n) | 0.579 | 0.133 | <0.001 | 0.379 | 0.092 | 0.004 |
| Plasma adiponectin | −0.422 | 0.148 | 0.020 | – | – | – |
| AHI | 0.311 | 0.192 | 0.030 | – | – | – |
| Adiponectin-positive hepatocytes (%) | −0.410 | 0.197 | 0.010 | – | – | – |
| Hepatocyte apoptotic index | 0.394 | 0.136 | 0.029 | – | – | – |
| Predictors of fibrosis stage | ||||||
| SaO2 <90% (%TST) | 0.651 | 0.102 | <0.001 | 0.358 | 0.123 | 0.004 |
| Plasma LPS | 0.602 | 0.118 | <0.001 | 0.319 | 0.114 | 0.007 |
| TLR-4-positive HSC (n) | 0.528 | 0.162 | <0.001 | 0.299 | 0.091 | 0.009 |
| BMI z-score | 0.412 | 0.173 | 0.021 | – | – | – |
| AHI | 0.295 | 0.111 | 0.034 | – | – | – |
| TLR-4-positive Kupffer cells (n) | 0.381 | 0.112 | 0.029 | – | – | – |
| HSC (n) | 0.421 | 0.113 | 0.019 | – | – | – |
| Adiponectin-positive HPCs (n) | −0.422 | 0.152 | 0.020 | – | – | – |
Steatosis score, hepatocyte ballooning score, lobular inflammatory score, NAS and fibrosis stage were entered as continuous variables into a multiple linear regression model.
AHI, apnoea/hypopnoea index; BMI, body mass index; ETpCO2, mean end-tidal pCO2; HPC, hepatic progenitor cell; HSC, hepatic stellate cell; L/M ratio, lactulose/mannitol ratio; LPS, lipopolysaccharide; NAFLD, non-alcoholic fatty liver disease; NAS, NAFLD activity score; ODI, oxygen desaturation index; SaO2 <90%, total duration of haemoglobin desaturation, expressed as %TST; TLR-4, toll-like receptor-4.