Univariable (left panel) and multivariable (right panel) linear regression analysis of the predictors of different hepatic immunohistochemical and immunofluorescence parameters in the whole NAFLD population (n=80)
| Univariable | Multivariable | |||
|---|---|---|---|---|
| Parameter | β (SE (β)) | p Value | β (SE (β)) | p Value |
| Hepatocyte cell-cycle arrest index | ||||
| SaO2 <90% (%TST) | 0.442 (0.131) | 0.002 | 0.442 (0.131) | 0.002 |
| Plasma LPS (EU/mL) | 0.389 (0.160) | 0.002 | – | – |
| HPCs (n) | 0.221 (0.112) | 0.039 | – | – |
| TLR-4-positive hepatocytes (%) | 0.228 (0.139) | 0.028 | – | – |
| Hepatocyte apoptotic index | ||||
| SaO2 <90% (%TST) | 0.423 (0.101) | <0.001 | 0.397 (0.134) | 0.010 |
| Plasma CK18 fragments (U/L) | 0.378 (0.113) | 0.001 | – | – |
| Plasma LPS (EU/mL) | 0.351 (0.109) | 0.010 | – | – |
| HPCs (n) | 0.271 (0.103) | 0.039 | – | – |
| TLR-4-positive hepatocytes (%) | 0.220 (0.127) | 0.031 | – | – |
| TLR-4-positive hepatocytes (%) | ||||
| SaO2 <90% (%TST) | 0.695 (0.110) | <0.001 | 0.339 (0.112) | 0.002 |
| Plasma LPS(EU/mL) | 0.281 (0.112) | 0.034 | – | – |
| Hepatocyte apoptotic index | 0.304 (0.118) | 0.015 | – | – |
| Adiponectin-positive hepatocytes (%) | −0.290 (0.103) | 0.030 | – | – |
| ETpCO2 (mm Hg) | −0.221 (0.115) | 0.029 | – | – |
| TLR-4-positive Kupffer cells (n) | 0.371 (0.134) | 0.002 | – | – |
| TLR-4-positive hepatic Kupffer cells (n) | ||||
| SaO2<90% (%TST) | 0.655 (0.135) | <0.001 | 0.357 (0.135) | 0.006 |
| BMI z-score | 0.238 (0.104) | 0.026 | – | – |
| Plasma LPS(EU/mL) | 0.322 (0.128) | 0.012 | – | – |
| AHI (events/h) | 0.218 (0.101) | 0.030 | – | – |
| Nadir SaO2 (%) | 0.239 (0.075) | 0.025 | – | – |
| Hepatic TLR-4-positive hepatocytes (%)* | 0.296 (0.128) | 0.029 | – | – |
| TLR-4-positive HSCs (n) | 0.358 (0.111) | 0.009 | – | – |
| Hepatic Kupffer cells (n) | 0.293 (0.112) | 0.029 | – | – |
| TLR-4-positive HSCs (n) | ||||
| SaO2 <90% (%TST) | 0.675 (0.137) | <0.001 | 0.445 (0.137) | 0.002 |
| BMI z-score | 0.278 (0.101) | 0.021 | – | – |
| Plasma hyaluronic acid (ng/mL) | 0.348 (0.102) | 0.010 | – | – |
| Plasma LPS (EU/mL) | 0.399 (0.114) | 0.008 | – | – |
| AHI (events/h) | 0.245 (0.103) | 0.021 | – | – |
| Hepatic TLR-4-positive Kupffer cells (n)* | 0.239 (0.121) | 0.029 | – | – |
| HSCs (n)* | 0.387 (0.103) | 0.007 | – | – |
| HPCs (n) | 0.298 (0.133) | 0.018 | – | – |
| HPCs (n) | ||||
| SaO2 <90%(%TST) | 0.650 (0.112) | <0.001 | 0.471 (0.191) | 0.001 |
| Plasma LPS(EU/mL) | 0.291 (0.131) | 0.021 | – | – |
| AHI (events/h) | 0.279 (0.116) | 0.034 | – | – |
| Hepatocyte cell-cycle arrest index (%) | 0.346 (0.153) | 0.010 | – | – |
| Hepatocyte apoptotic index (%) | 0.339 (0.123) | 0.011 | – | – |
| TLR-4-positive hepatic stellate cells (n) | 0.396 (0.118) | 0.018 | – | – |
| Adiponectin-positive HPCs (n) | ||||
| SaO2 <90% (%TST) | −0.698 (0.119) | <0.001 | −0.532 (0.141) | <0.001 |
| Plasma LPS (EU/mL) | 0.319 (0.118) | 0.013 | – | – |
| AHI (events/h) | 0.272 (0.114) | 0.034 | – | – |
| Hepatocyte cell-cycle arrest index (%) | −0.345 (0.125) | 0.019 | – | – |
| Hepatocyte apoptotic index (%) | −0.299 (0.103) | 0.030 | – | – |
| Adiponectin-positive hepatocytes (%) | 0.436 (0.112) | 0.001 | – | – |
Only statistically significant associations are shown. Skewed variables were log-transformed.
AHI, apnoea/hypopnoea index; BMI, body mass index; ETpCO2, mean end-tidal pCO2; HPC, hepatic progenitor cell; HSC, hepatic stellate cell; L/M ratio, lactulose/mannitol ratio; LPS, lipopolysaccharide; NAFLD, non-alcoholic fatty liver disease; ODI, oxygen desaturation index; SaO2<90%, total duration of haemoglobin desaturation, expressed as %TST; TLR-4, toll-like receptor-4.