Table 3

Clonality assessment over time in patients 2, 3, 4 and 5

Patient	Bronchoscopy date	Time post-lobectomy (months)	Biopsy site	Diagnosis	Mutated gene (exon)	Mutation identified
2	07/2001	M₃₀	MC (control)	NAD	–	Wildtype
	08/2002	M₄₃	LUL	SD	TP53-7	p.C242F; c.725G>T
	08/2002	M₄₃	LLL stump	SD	TP53-7	p.C242F; c.725G>T
	12/2002	M₄₇	MC	MoD	TP53-7	p.C242F; c.725G>T
			MC	Metaplasia	–	Wildtype
			LUL/lingula sub	CIS	TP53-7	p.C242F; c.725G>T
	03/2003	M₅₀	RLL segmentectomy	CA	KRAS	p.E88*; c.262G>T
	02/2006	M₈₅	Trachea	CA	TP53-7	p.C242F; c.725G>T
3	05/2003	M₃	MC (control)	NAD	–	Wildtype
	10/2003	M₈	LUL stump	SD	TP53-5	p.V173L; c.517G>T
	08/2005	M₃₀	Left pneumonectomy stump	SD/CIS		p.V173L; c.517G>T
	03/2006	M₃₇	Trachea	CIS		p.V173L; c.517G>T
	08/2006	M₄₂	Left pneumonectomy stump	SqCC		p.V173L; c.517G>T
	08/2006	M₄₂	Lower trachea	SqCC		p.V173L; c.517G>T
4	07/2009	M₉	Proximal RMB	CIS	TP53-5	p.V173L; c.517G>T p.V157F; c.469G>T
	09/2009	M₁₁	RUL stump	SqCC	TP53-5	p.V173L; c.517G>T p.V157F; c.469G>T
	09/2009	M₁₁	Posterior wall lower trachea	SqCC	TP53-5	p.V173L; c.517G>T
5	06/2004	M₅₀	LLL (control)	NAD	–	Wildtype
			LUL (control)	NAD	–	Wildtype
			Right lobectomy stump	MoD/SD	TP53-7	p.C242R; c.724T>C p.G244D; c.731G>A
	11/2004	M₅₅	Right lobectomy stump	SD		p.C242R; c.724T>C p.G244D; c.731G>A
	04/2005	M₆₀	RIB	CIS/Inv.		p.C242R; c.724T>C p.G244D; c.731G>A
	04/2005	M₆₀	Lower trachea	CIS		p.C242R; c.724T>C p.G244D; c.731G>A
	10/2005	M₆₆	RMB polyp	SD		p.C242R; c.724T>C p.G244D; c.731G>A

CIS, carcinoma in situ; LLL, left lower lobe; LUL, left upper lobe; RIB, right intermediate bronchus; RLL, right lower lobe; RMB, right main bronchus; RUL, right upper lobe; SD, severe dysplasia.