Table 2

Tracing the spread of the TP53 p.E294fs*51 (c.880delG) mutant clone over time in patient 1

AFB date	Month	Biopsy site	Diagnosis	AFB status	TP53 status	LoH/RoH (D17S 1881)
10/2000	M₃₈	LUL stump	CIS	+	Mutant	LoH
		LLL orifice (control)	NAD	−	Wildtype	RoH
		LLL (control)	NAD	−	Wildtype	RoH
		*MC (control)	NAD	−	Wildtype	RoH/RoH
		RUL (control)	NAD	−	Wildtype	RoH
		RLL (control)	NAD	−	Wildtype	RoH
03/2001	M₄₃	LUL stump	SD	+	Mutant	LoH
07/2001	M₄₇	LUL stump	CIS	+	Mutant	LoH
12/2001	M₅₁	LLL (control)	NAD	−	Wildtype	RoH
01/2003	M₆₄	Left main bronchus	SD	+	Mutant	N/A
07/2003	M₇₀	LLL (control)	NAD	−	Wildtype	RoH
		†LLL orifice	SD	+	Mutant	LoH /LoH
		Proximal left main bronchus	SD	+	Mutant	LoH
		Left main bronchus	CIS	+	Mutant	N/A
11/2003	M₇₄	LLL (control)	NAD	−	Wildtype	N/A
11/2003	M₇₄	Proximal left main bronchus	CIS	+	Mutant	N/A
05/2004	M₈₀	LLL (control)	NAD	−	Wildtype	N/A
09/2004	M₈₄	LLL (control)	NAD	−	Wildtype	N/A
04/2006	M₁₀₄	MC	CIS	+	Mutant	N/A
04/2006	M₁₀₄	MC (control)	NAD	−	Wildtype	N/A
08/2006	M₁₀₈	Lower anterior trachea	CIS	+	Mutant	LoH
01/2007	M₁₁₃	Lower lateral trachea	CIS	+	Mutant	LoH
04/2007	M₁₁₆	Lower trachea	CIS	+	Mutant	N/A
03/2008	M₁₂₇	RMB (control)	NAD	−	Wildtype	N/A
		RMB	MoD	+	Mutant	N/A
		Lower trachea (control)	NAD	−	Wildtype	RoH
08/2008	M₁₃₂	MC/stump	CIS	+	Mutant	N/A
		Proximal RMB medial wall	CIS	+	Mutant	LoH
		Proximal RMB medial wall	SD	+	Mutant	N/A
01/2009	M₁₃₇	RMB	CIS	+	Mutant	N/A
01/2009	M₁₃₇	LMB stump	CIS	+	Mutant	LoH
07/2009	M₁₄₃	Lateral wall of orifice to RMB	CIS	+	Mutant	LoH
		Posterior wall proximal RMB	CIS	+	Mutant	LoH
		Left pneumonectomy stump	CIS	+	Mutant	LoH
11/2010	M₁₅₉	RMB	MiD	−	Wildtype	RoH
		RMB	Metaplasia	−	Wildtype	RoH
		MC	CIS	+	Mutant	LoH
		MC	MiD	−	Wildtype	RoH
		MC (control)	NAD	−	Wildtype	RoH
		RML (control)	NAD	−	Wildtype	RoH
05/2011	M₁₆₅	RML	CIS	+	Mutant	N/A
03/2012	M₁₇₅	RML	CIS	+	Mutant	N/A
03/2012	M₁₇₅	RLL	CIS	+	Mutant	N/A
08/2012	M₁₈₀	Posterior wall of RMB	SqCC	+	Mutant	N/A

Month refers to post-lobectomy.
*At M₃₈, two distinct areas of the MC were biopsied, providing two AFB and histology normal samples.
†At M₇₀, two distinct AFB detected and histology-confirmed SD samples from the LLL orifice were analysed and found to carry the c.880delG. In total, 46 samples from 19 spatially distinct areas were analysed over a period of 12 years (M₃₈–M₁₈₀).
AFB, auto-fluorescence bronchoscopy; CIS, carcinoma in situ; LLL, left lower lobe; LMB, left main bronchus; LoH, loss of heterozygosity; LUL, left upper lobe; MiD, mild dysplasia; MoD, moderate dysplasia; N/A, not analysed due to insufficient DNA lysate; NAD, no abnormality detected; RLL, right lower lobe; RMB, right main bronchus; RML, right middle lobe; RoH, retention of heterozygosity; RUL, right upper lobe; SD, severe dysplasia.