Table 2

List of 50 genes that changed significantly after both 6 and 30 months of treatment with fluticasone±salmeterol and could be confirmed in the fourth GLUCOLD treatment arm by both a change in the same direction after 6 months fluticasone treatment and a change in the opposite direction after fluticasone withdrawal

Gene symbol	Change after 6 and 30 months of treatment with fluticasone±salmeterol versus placebo				Change after 6 months fluticasone treatment and after fluticasone withdrawal between 6 and 30 months
	0–6 months fluticasone±salmeterol versus placebo		0–30 months fluticasone±salmeterol versus placebo		0–6 months fluticasone treatment versus placebo		6–30 months fluticasone withdrawal versus placebo
	Fold change	p Value	Fold change	p Value	Fold change	p Value	Fold change	p Value
TMPRSS11D	−1.959	<0.001	−1.842	0.001	−1.746	0.006	1.341	0.030
SERPINB13	−2.126	0.001	−1.686	0.041	−1.919	0.023	1.432	0.035
SPINK5	−2.200	0.001	−1.631	0.032	−1.902	0.013	1.424	0.031
KRT4	−3.592	<0.001	−1.554	0.030	−3.485	0.003	1.711	0.002
TMPRSS11A	−3.329	<0.001	−1.552	0.014	−1.125	<0.001	2.092	<0.001
CSTA	−2.292	<0.001	−1.485	0.016	−2.259	0.004	1.567	0.008
GABRP	−1.624	0.002	−1.440	0.007	−1.660	<0.001	1.260	0.017
TYMS	−1.930	<0.001	−1.417	0.023	−1.802	0.002	1.372	0.005
GPR87	−1.723	<0.001	−1.375	0.017	−1.619	0.007	1.230	0.025
TMPRSS4	−1.816	<0.001	−1.358	0.027	−1.655	0.007	1.279	0.009
ATP10B	−1.498	0.003	−1.358	0.023	−1.437	0.017	1.299	0.033
CH25H	−1.531	0.004	−1.335	0.023	−1.601	0.001	1.476	0.004
SRPX2	−1.723	<0.001	−1.317	0.022	−1.643	0.010	1.341	0.025
IGKC	−1.603	0.002	−1.312	0.045	−1.533	0.006	1.792	0.016
BNIPL	−1.501	0.002	−1.291	0.012	−1.532	<0.001	1.141	0.033
RAB38	−1.522	0.001	−1.281	0.025	−1.497	0.002	1.167	0.033
CAPNS2	−1.707	<0.001	−1.275	0.040	−1.708	<0.001	1.276	0.023
FANCD2	−1.443	<0.001	−1.261	0.014	−1.323	0.003	1.137	0.044
ABCC1	−1.529	<0.001	−1.241	0.026	−1.418	0.009	1.271	0.005
ODZ2	−1.504	0.001	−1.237	0.029	−1.425	0.023	1.295	0.034
BNC1	−1.495	<0.001	−1.233	0.027	−1.406	0.028	1.178	0.028
KLK10	−1.464	0.008	−1.221	0.037	−1.429	0.013	1.289	0.027
PTAFR	−1.366	0.002	−1.218	0.025	−1.284	0.008	1.210	0.006
CRABP2	−1.680	<0.001	−1.213	0.034	−1.582	0.008	1.568	<0.001
ODZ4	−1.281	0.001	−1.169	0.015	−1.311	0.001	1.169	0.016
TRIM16	−1.496	<0.001	−1.167	0.035	−1.594	0.001	1.244	0.033
EYA2	−1.213	0.010	−1.160	0.021	−1.187	0.034	1.121	0.021
GNA15	−1.555	<0.001	−1.154	0.048	−1.474	0.013	1.191	0.028
SMAGP	−1.422	<0.001	−1.152	0.019	−1.383	0.002	1.210	0.002
BICD2	−1.309	<0.001	−1.145	0.026	−1.240	0.022	1.170	0.014
EXOSC7	−1.211	0.001	−1.141	0.027	−1.156	0.015	1.103	0.015
SCO1	−1.158	0.013	−1.134	0.037	−1.175	0.020	1.083	0.006
BID	−1.171	0.011	−1.132	0.039	−1.168	0.015	1.125	0.046
C12orf32	−1.213	0.001	−1.122	0.025	−1.253	0.005	1.113	0.035
ITPA	−1.293	<0.001	−1.108	0.029	−1.212	0.021	1.169	0.005
TSPAN17	−1.259	<0.001	−1.102	0.025	−1.201	0.015	1.208	0.004
FKBP5	1.931	<0.001	2.284	<0.001	1.791	<0.001	−1.389	0.011
PDK4	1.689	0.001	1.719	0.002	1.726	<0.001	−1.486	0.014
RHOBTB3	1.469	<0.001	1.519	0.003	1.511	<0.001	−1.629	0.003
ART3	1.570	0.003	1.411	0.016	1.446	0.015	−1.235	0.019
PPM1K	1.397	<0.001	1.326	0.004	1.285	<0.001	−1.446	0.004
HIF3A	1.342	0.007	1.304	0.005	1.248	0.039	−1.116	0.034
KLF9	1.217	0.020	1.292	<0.001	1.276	0.003	−1.084	0.037
SLC39A8	1.319	0.002	1.238	0.033	1.399	<0.001	−1.265	0.015
PDE4DIP	1.331	0.001	1.236	0.010	1.291	0.003	−1.192	0.021
PHF17	1.325	<0.001	1.233	0.011	1.249	0.001	−1.305	0.001
KLF15	1.340	0.003	1.217	0.019	1.299	0.008	−1.166	0.022
KCNAB1	1.386	0.001	1.216	0.038	1.347	0.009	−1.252	0.002
PRPH	1.201	0.005	1.178	0.010	1.174	0.017	−1.092	0.006
TMX4	1.167	0.003	1.111	0.047	1.176	0.013	−1.270	0.001