Table 2 Oligonucleotide sequences of primers used to amplify GAPDH (housekeeping), TGFβ and SCCA

Primer	Sequence 5′–3′	Annealing temp (°C)	Amplicon size (bp)
GAPDH Fw	GGGCTCTCCAGAACATCATCC	60	130 bp
GAPDH Rv	GTCCACCACTGACACGTTGG	60	130 bp
SCCA Fw	GGCAGCTGCAGCTTCTG	55	80 bp
SCCA Rv	AGCCGCGGTCTCGTGC	55	80 bp
TGFβ Fw	GCCCTGGACACCAACTATTGC	60	161 bp
TGFβ Rv	AGGCTCCAAATGTAGGGGCAG	60	161 bp

bp, base pair; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Fw, forward; Rv, reverse; SCCA, squamous cell carcinoma antigen; TGFβ, transforming growth factor β.