Table 2

Antibodies used to characterise inflammatory cells and CXC, CC chemokines, their receptors and other molecules involved in the neutrophilia of the bronchial mucosa

Antibody specificityCompanyCatalogue numberSource/hostDilution of the primary antibodyBiotinylated secondary antibodyRevealing kitPositive control
CXCL1R&D SystemsAF-275Goat1:50Vector, BA 5000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCL5R&D SystemsMAB-254Mouse1:50Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCL6Santa CruzSC-5813Goat1:50Vector, BA 5000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCL7R&D SystemsMAB393Mouse1:50Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCL8R&D SystemsAF-208NAGoat1:50Vector, BA 5000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CCL5R&D SystemsAF-278NAGoat1:100Vector, BA 5000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCR1R&D SystemsMAB330Mouse1:200Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CXCR2R&D SystemsMAB331Mouse1:200Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CD44AMS-Biotech211-JM144AMouse1:100Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
CD11bR&D SystemsMAB16991Mouse1:50Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Nasal polyp
Neutrophil elastaseDakoM752Mouse1:100Vector, BA 2000; 1:200Dako, StreptABComplex/AP; 1:100Human tonsil