Table 2

 Recommended culture conditions for CF pathogens from respiratory samples

BacteriaRecommended media condition*Special conditions
*Media listed are commercially available.
Staphylococcus aureus Mannitol salt agar
Columbia/colistin-nalidixic acid agar
Haemophilus influenzae Horse blood or chocolate agar (may be supplemented with 300 μg/ml bacitracin)Incubate anaerobically
Pseudomonas aeruginosa MacConkey agar
Burkholderia cepacia complexBCSA OFPBL agar PC agarEnhanced detection of slow-growing colonies by incubation up to 4 days
Stenotrophomonas maltophilia MacConkey agar VIA agarDNase agar used for confirmation
Achromobacter xylosoxidans MacConkey agar