Antibodies used for immunohistochemistry
| Antibody | Pretreatment | Species | Dilution | Clone | Origin* |
|---|---|---|---|---|---|
| *Dako, Glostrup, Denmark; Novocastra, Newcastle upon Tyne, UK; Pharmacia Diagnostics, Uppsala, Sweden; IQ Products, Groningen, The Netherlands; CLB/Sanquin, Amsterdam, The Netherlands. | |||||
| CD3 (lymphocyte) | Citrate | Rabbit | 1:500 | Polyclonal | Dako |
| CD4 (T helper) | Citrate | Mouse | 1:300 | CD4-368 | Novocastra |
| CD8 (T cytotoxic) | Citrate | Mouse | 1:50 | CD8-144B | Dako |
| Neutrophil elastase (neutrophil) | Protease | Mouse | 1:3200 | NP57 | Dako |
| CD68 (macrophage) | Citrate | Mouse | 1:3000 | KP1 | Dako |
| Tryptase (mast cell) | Citrate | Mouse | 1:16000 | AA1 | Dako |
| EG2 (eosinophil) | Trypsin | Mouse | 1:150 | EG-2 | Pharmacia |
| CD138 (plasma cell) | EDTA | Mouse | 1:6400 | B-B4 | IQ products |
| Granzyme B | Citrate | Mouse | 1:200 | CLB-GB7 | CLB/Sanquin |