Primer and probe sequences for SNPs tested in SFRS8
| Gene | SNP | SNP function | Primer | Sequence |
|---|---|---|---|---|
| *Underlined nucleotides in the sequence of probes used for allele discrimination on real-time PCR represent the polymorphic site. | ||||
| †MGB, Minor Groove Binder (Applied Biosystems). | ||||
| ‡SNP tested by Assays on Demand (Applied Biosystems) so the exact primer/probe sequence is only known by the company. | ||||
| ¶SNPs are exonic SNPs in the long transcript of SFRS8 (NM_004592). | ||||
| SFRS8 | rs1379049 | Upstream | PCR forward | 5′-cgccaccctgggcaga |
| PCR reverse | 5′-tgctgcagcctgccacat | |||
| Probe I | 5′-fam-cctccgcgtccctcaccatg-tamra* | |||
| Probe II | 5′-vic-agcctccgcgcccctcac-tamra | |||
| rs3782288 | Intron | PCR forward | 5′-tgagtcaaaccatgtcctgcc | |
| PCR reverse | 5′-cgtggtgtccatgttagtggag | |||
| Probe I | 5′-fam-gcctagtcactaaaa-MGB† | |||
| Probe II | 5′-vic-gcctagtcactagaac-MGB | |||
| rs930863 | Intron | ‡ | ||
| rs1051219 | Synonymous codon change¶ | PCR forward | 5′-gaccgtggcagccatgtatta | |
| PCR reverse | 5′-ggtcgtcactccaggggagt | |||
| Probe I | 5′-fam-ccctcccggaatcgacgtgact-tamra | |||
| Probe II | 5′-joe-cccctcccggaatcgatgtgact-tamra | |||
| rs1107871 | Intron | ‡ | ||
| rs1051233 | Synonymous codon change¶ | PCR forward | 5′-ctggaagatcgcctcgca | |
| PCR reverse | 5′-tctgcttccggcagaggat | |||
| Probe I | 5′-fam-tgcccgggaaaagctggcc-tamra | |||
| Probe II | 5′-joe-tgcccgggaaaagctcgcc-tamra | |||
| rs755437 | Intron | ‡ | ||