Table 2

 Primer and probe sequences for SNPs tested in SFRS8

GeneSNPSNP functionPrimerSequence
*Underlined nucleotides in the sequence of probes used for allele discrimination on real-time PCR represent the polymorphic site.
†MGB, Minor Groove Binder (Applied Biosystems).
‡SNP tested by Assays on Demand (Applied Biosystems) so the exact primer/probe sequence is only known by the company.
¶SNPs are exonic SNPs in the long transcript of SFRS8 (NM_004592).
SFRS8 rs1379049UpstreamPCR forward5′-cgccaccctgggcaga
PCR reverse5′-tgctgcagcctgccacat
Probe I5′-fam-cctccgcgtccctcaccatg-tamra*
Probe II5′-vic-agcctccgcgcccctcac-tamra
rs3782288IntronPCR forward5′-tgagtcaaaccatgtcctgcc
PCR reverse5′-cgtggtgtccatgttagtggag
Probe I5′-fam-gcctagtcactaaaa-MGB†
Probe II5′-vic-gcctagtcactagaac-MGB
rs930863Intron
rs1051219Synonymous codon change¶PCR forward5′-gaccgtggcagccatgtatta
PCR reverse5′-ggtcgtcactccaggggagt
Probe I5′-fam-ccctcccggaatcgacgtgact-tamra
Probe II5′-joe-cccctcccggaatcgatgtgact-tamra
rs1107871Intron
rs1051233Synonymous codon change¶PCR forward5′-ctggaagatcgcctcgca
PCR reverse5′-tctgcttccggcagaggat
Probe I5′-fam-tgcccgggaaaagctggcc-tamra
Probe II5′-joe-tgcccgggaaaagctcgcc-tamra
rs755437Intron