Studies with quantitative or semi-quantitative assessment of iNOS expression in cystic fibrosis
| Study | Cell source | CF:controls(n) | Age (years) CF:controls | Nature of controls | Outcomes | iNOS expression |
|---|---|---|---|---|---|---|
| CFBE41, CF airway epithelial cell line 41; 16HBE, human bronchial epithelial cell line; IHC, immunohistochemistry; PCR, polymerase chain reaction; Western: western-blot; NO: nitric oxide. | ||||||
| *11/17 CF and 9/14 non-CF samples allowed ICH analysis of NOS expression. | ||||||
| †Five samples from explanted non-CF lungs and four samples by bronchoscopic brushing of healthy volunteers. | ||||||
| Wooldridge13 | Ex vivo bronchoscopy | 17:14* | 6.5:3.5 | Respiratory disease or symptoms | Semi-quantitative IHC | No difference |
| Darling21 | CFBE41 | – | – | 16HBE | Chemiluminescence | Low NO production |
| Meng14 | CFBE41 | – | – | 16HBE | PCR, Western | Reduced |
| Meng9 | CFBE41 biopsy | 13:14 | >19 | 16HBE + emphysema | PCR, Western | Reduced |
| Kelley8 | Murine transgenic line | Non-CF mice | PCR, Western | Reduced | ||
| Morrissey22 | Ex vivo lung sections | 5:3 | 21–42:50–68 | Lung cancer | Semi-quantitative IHC | Reduced |
| Dotsch6 | Ex vivo nasal polyps | 3:4 | 13–18:40–59 | Non-CF nasal polyps | PCR | Reduced |
| Steagall23 | Murine CFTR knock out | – | – | CFTR+/+ mice | IHC, PCR, Iso NO-meter | Reduced to absent |
| Kahn24 | Ex vivo lung sections | 7:9† | – | Healthy + non-CF lung disease | PCR, Western IFN-γ stimulation | Reduced iNOS promoter activity |