RT-PCR conditions
| mRNA of interest | Sequences | Annealings temperature(°C) | No of cycles | Product length(bp) |
|---|---|---|---|---|
| GAPDH | Sense 5′-CGT CTT CAC CAC CAT GGA GA-3′ | 55 | 26 | 330 |
| Antisense 5′-CGG CCA TCA CGC CAC AGT TT-3′ | ||||
| β-actin | Sense 5′-TCA-CCC-ACA-CTG-TGC-CCA-TCT-ACG-A-3′ | 62 | 25 | 294 |
| Antisense 5′-CAG-CGG-AAC-CGC-TCA-TTG-CCA-ATG-G-3′ | ||||
| HAS2 | Sense 5′-CCT GGG CTA TGC AAC AAA AT -3′ | 52 | 36 | 314 |
| Antisense 5′-TAA GGC AGC TGG CAA AAG AT-3′ | ||||
| HYAL2 | Sense 5′-GTG GTG TGC CAC AGA ATG TC-3′ | 52 | 36 | 330 |
| Antisense 5′-AGA GGT AGA AGC CCC AGA GG-3′ |