Table 3

 Genes regulated by several stimuli in neutrophils of healthy donors (n = 2)

DescriptionGenbank numberTNFα(fold change)GM-CSF(fold change)TNFα + GM-CSF(fold change)
TNFα, tumour necrosis factor α; GM-CSF, granulocyte-macrophage colony stimulating factor; IL, interleukin; IL-8Rα, IL-8 receptor α; IL-1Rβ, IL-1 receptor β; IL-1RA, IL-1 receptor antagonist; MIP, macrophage inflammatory protein.
Neutrophils were incubated with TNFα (100 U/ml) and/or GM-CSF (0.1 nM) for 3 hours in vitro. RNA was isolated and used for generating radioactively labelled cDNA which was hybridised with Atlas array filters. Membranes were analysed using PhosphorImager and Image Quant software. Changes in gene expression are relative to control.
Chemokine/cytokine receptors
Blood disorder proteins
    Antileukoproteinase (ALP) 1X044705.1
    Retinoic acid receptor α (RAR-α)M7377915.0
    B cell translocation gene 1 (BTG1)X61123−10.3−9.2
    NOTCH 1M73980−4.5−6.4−5.8
Surface antigens
    CD14 antigenM865112.89.54.5
    CD44 antigenM590408.721.616.6
    CD58 antigenY006363.46.73.5
    CD83 antigenZ1169712.510.3
    CD69 antigenL0755517.48.2
Transcription factors
    fli1 proto-oncogeneM93255−3.1−6.9−16.3
Protein kinases/oncogenes
    ras-like small GTPase TTFZ352278.2
    BCL2-related protein A1 (BCL2-A1)U2968013.94.719.2