Table 3

 Genes regulated by several stimuli in neutrophils of healthy donors (n = 2)

DescriptionGenbank numberTNFα(fold change)GM-CSF(fold change)TNFα + GM-CSF(fold change)
TNFα, tumour necrosis factor α; GM-CSF, granulocyte-macrophage colony stimulating factor; IL, interleukin; IL-8Rα, IL-8 receptor α; IL-1Rβ, IL-1 receptor β; IL-1RA, IL-1 receptor antagonist; MIP, macrophage inflammatory protein.
Neutrophils were incubated with TNFα (100 U/ml) and/or GM-CSF (0.1 nM) for 3 hours in vitro. RNA was isolated and used for generating radioactively labelled cDNA which was hybridised with Atlas array filters. Membranes were analysed using PhosphorImager and Image Quant software. Changes in gene expression are relative to control.
Chemokine/cytokine receptors
    IL-8RαM68932–8.6−6.4
    IL-1RβX5977011.513.64.4
Blood disorder proteins
    Antileukoproteinase (ALP) 1X044705.1
    Retinoic acid receptor α (RAR-α)M7377915.0
    B cell translocation gene 1 (BTG1)X61123−10.3−9.2
    NOTCH 1M73980−4.5−6.4−5.8
Chemokines/cytokines
    IL-1βK0277013.027.3
    IL-1RAM63099106.798.5227.6
    IL-8Y007877.19.322.2
    MIP-1βJ041306.04.1
    Lymphotoxin-βL110153.2
Surface antigens
    ICAM-1J0313215.45.018.1
    CD14 antigenM865112.89.54.5
    CD44 antigenM590408.721.616.6
    CD58 antigenY006363.46.73.5
    CD83 antigenZ1169712.510.3
    CD69 antigenL0755517.48.2
Transcription factors
    MNDAM81750−4.4−11.8
    STAT5A/BU47686−5.7
    fli1 proto-oncogeneM93255−3.1−6.9−16.3
Protein kinases/oncogenes
    vav2S7699212.010.7
    SLP-76U201583.315.215.1
    ras-like small GTPase TTFZ352278.2
Apoptosis
    BCL2-related protein A1 (BCL2-A1)U2968013.94.719.2