Table 1

Primer sequences, genomic localisation, cycle profiles, and the expected length of the PCR product

Virus	Primer	Primer sequence 5`- 3` direction	Genomic position	Cycle profile (40×)	Length of PCR product (bp)
*HRV and enterovirus are referred to as picornaviruses.
RSV²⁸	G1	CCA TTC TGG CAA TGA TAA TCT C	Glycoprotein gene	55°C/1min – 72°C/2min – 95°C/1min
	G2	GTT TTT TGT TTG GTA TTC TTT TGC AG
	G3	CGG CAA ACC ACA AAG TCA CAC		50°C/1min – 72°C/2min – 95°C/1min	326
	G4	GGG TAC AAA GTT AAA CAC TTC

Influenza A	Anp1	ATC ACT CAC TGA GTG ACA TC	Nucleoprotein gene	55°C/1min – 72°C/2min – 95°C/1min
	Anp2	CCT CCA GTT TTC TTA GGA TC
	Anp3	CAT GGC GTC CCA AGG CAC C		55°C/1min – 72°C/2min – 95°C/1min	271
	Anp4	CCT AGG ATC CTT CCC CGC

Influenza B	Bns1	CGG ACA ACA TGA CCA CAA C	Non-structural gene	50°C/1min – 72°C/2min – 95°C/1min
	Bns2	TCT CTC TTC AAG AGA CAT CC
	Bns3	CCA CAA CAC AAA TTG AGG TG		50°C/1min – 72°C/2min – 95°C/1min	201
	Bns4	CAT CCT TTT ACT TTC AGG

PIV 3	PIV 3-1	AGA AGT CAA TAC CAA CAA CTA	Fusion protein gene	50°C/1min – 72°C/2min – 95°C/1min
	PIV 3-4	GGC AGA AAG ATG CCA TAA TCA TGG
	PIV 3-2	GCA GTA TTG AGG TTG GCA TGA TG		55°C/1min – 72°C/2min – 95°C/1min	183
	PIV 3-3	TTA GCA GCC ACA CTC GCT GG

HRV*	Rh1	CGG ACA CCC AAA GTA A	Non-translated region	44°C/1min – 72°C/2min – 95°C/1min
	Rh2	GCA CTT CTG TTT CCC C
	Rh2	GCA CTT CTG TTT CCC C		44°C/1min – 72°C/2min – 95°C/1min	204
	Rh3	GGC AGC CAC GCA GGC T

Enterovirus*	Rh1	CGG ACA CCC AAA GTA A	Polyprotein gene	44°C/1min – 72°C/2min – 95°C/1min
	Rh2	GCA CTT CTG TTT CCC C
	Rh2	GCA CTT CTG TTT CCC C		44°C/1min – 72°C/2min – 95°C/1min	297
	PV4	GCA TTC AGG GGC CGG AG