Details of primers used
| mRNA | Primer sequence | Annealing temperature (°C) | PCR product size (bp) | ||||
|---|---|---|---|---|---|---|---|
| Native | Competitor | ||||||
| IL-2 | Upper: GAATGGAATTAATAATTACAAGAATCC Lower: TGTTTCAGATCCCTTTAGTTCCAG | 56 | 229 | 305 | |||
| IL-4 and IL-4δ2 | Upper: CCGGCAACTTTGTCCAC Lower: TCTGGTTGGCTTCCTTCAC | 64 | 339 and 291 | 499 | |||
| IL-5 | Upper: GATAGCCAATGAGACTCTGAGG Lower: TTGCCTGGAGGAAAATACTT | 52 | 493 | 409 | |||
| IFNγ | Upper:CGTTTTGGGTTCTCTTGGCTGTT Lower:CTCCTTTTTCGCTTCCCTGTTTT | 60 | 423 | 570 | |||
| IL-5 mutagenesis | Composite upper:2-150 GATAGCCAATGAGACTCTGAGG- TGGCTGTGCCTATTTCTATG Lower: TTGCCTGGAGGAAAATACTT | 55 | – | 409 | |||
↵2-150 Sequence identical to IL-5 upper primer is indicated by underlining.