Table 2

Bacteria detected by culture and molecular analysis (terminal restriction fragment length polymorphism, T-RFLP) in sputum samples (n=64) collected from adult patients with cystic fibrosis

Number of samples in which genera detected by:
GeneraCultureT-RFLP*
Achromobacter13
Actinomyces233
Atopobium61
Bacillus2ND
Burkholderia1716
Corynebacterium11
Devosia02
Finegoldia1ND
Fusobacterium14
Gemella230
Granulicatella045
Haemophilus06
Lactobacillus8ND
Micrococcus6ND
Neisseria01
Pasteuriaceae incerta05
Pediococcus2ND
Planomicrobium02
Porphyromonas06
Prevotella187
Propionibacterium31
Pseudomonas4038
Ralstonia03
Rothia52
Staphylococcus201
Stenotrophomonas1ND
Streptococcus1947
Variovorax2ND
Veillonella3327
Total211251
  • * Genera only included if they were detected by T-RFLP with a threshold score of >0.2 (see online supplement).

  • Genera for which no T-RFLP reference spectra were generated and therefore could not be detected (ND) by T-RFLP analysis.