RT Journal Article SR Electronic T1 SARS-CoV-2 environmental contamination from hospitalised patients with COVID-19 receiving aerosol-generating procedures JF Thorax JO Thorax FD BMJ Publishing Group Ltd and British Thoracic Society SP 259 OP 267 DO 10.1136/thoraxjnl-2021-218035 VO 77 IS 3 A1 Rebecca L Winslow A1 Jie Zhou A1 Ella F Windle A1 Intesar Nur A1 Ranjit Lall A1 Chen Ji A1 Jonathan Edward Millar A1 Paul M Dark A1 Jay Naisbitt A1 Anita Simonds A1 Jake Dunning A1 Wendy Barclay A1 John Kenneth Baillie A1 Gavin D Perkins A1 Malcolm Gracie Semple A1 Daniel Francis McAuley A1 Christopher A Green YR 2022 UL http://thorax.bmj.com/content/77/3/259.abstract AB Background Continuous positive airways pressure (CPAP) and high-flow nasal oxygen (HFNO) are considered ‘aerosol-generating procedures’ in the treatment of COVID-19.Objective To measure air and surface environmental contamination with SARS-CoV-2 virus when CPAP and HFNO are used, compared with supplemental oxygen, to investigate the potential risks of viral transmission to healthcare workers and patients.Methods 30 hospitalised patients with COVID-19 requiring supplemental oxygen, with a fraction of inspired oxygen ≥0.4 to maintain oxygen saturation ≥94%, were prospectively enrolled into an observational environmental sampling study. Participants received either supplemental oxygen, CPAP or HFNO (n=10 in each group). A nasopharyngeal swab, three air and three surface samples were collected from each participant and the clinical environment. Real-time quantitative polymerase chain reaction analyses were performed for viral and human RNA, and positive/suspected-positive samples were cultured for the presence of biologically viable virus.Results Overall 21/30 (70%) participants tested positive for SARS-CoV-2 RNA in the nasopharynx. In contrast, only 4/90 (4%) and 6/90 (7%) of all air and surface samples tested positive (positive for E and ORF1a) for viral RNA respectively, although there were an additional 10 suspected-positive samples in both air and surfaces samples (positive for E or ORF1a). CPAP/HFNO use or coughing was not associated with significantly more environmental contamination than supplemental oxygen use. Only one nasopharyngeal sample was culture positive.Conclusions The use of CPAP and HFNO to treat moderate/severe COVID-19 did not appear to be associated with substantially higher levels of air or surface viral contamination in the immediate care environment, compared with the use of supplemental oxygen.All data relevant to the study are included in the article or uploaded as supplementary information.